正交试验优选复方金沙利胆颗粒的提取工艺

Extraction Process Optimization of Fufang Jinsha Lidan Granule by Orthogonal Experiments

目的：研究探讨复方金沙利胆颗粒的最佳提取工艺条件。方法：根据处方中药所含主要成分的性质，通过正交试验对提取条件进行了筛选优化。以芍药苷含量和浸膏得率作为考察评价指标，选择加水量，煎煮时间，煎煮次数作为考察的三个影响因素进行优选。每因素设置3个水平，以L9（3^4）正交表进行正交试验。结果：通过正交试验确定了复方金沙利胆颗粒的最佳工艺为加12倍水（第一次提取加14倍水，浸泡30min），提取2次，每次煎煮1h。结论：该工艺提取率高，稳定性好，操作简单，浸膏得率较高，质量可控，值得推广应用。

Objective： To optimize the extraction technology of Fufang Jinsha Lidan granule. Methods： According to the properties of traditional Chinese medicines in the formula, orthogonal experiments were used to optimize the extraction process of Fufang Jinsha Li- dan granule. The volume of water, boiling time and boiling frequency were used as the three influencing factors with three different lev- els in the orthogonal experiments. Moreover, the content of paeoniflorin and the yield of extract were chosen as the evaluation indices. The orthogonal experiments were carried out according to the L9 （ 3^4 ） orthogonal table. Results ： The optimal extraction process of Fu- fang Jinsha Lidan granule was as follows： boiled twice, and one hour per time with 12-fold amount of water （ soaked for 30 rain with 14- fold amount of water for the first extraction process）. Conclusion： The extraction process has such properties as high extraction rate, stability, simple operation, high yield of extract and controllable quality, which is worthy of wide application.