HPLC法同时测定复方青蒿安乃近片中两成分的含量

Simultaneous Determination of Two Components in Compound Artemisinin Analgin Tablets

目的:建立HPLC法同时测定复方青蒿安乃近片中两成分的含量。方法:色谱柱为Waters Symmetry C18(250 mm×4.6 mm,5μm);流动相为甲醇-0.02 mol·L^(-1)磷酸二氢钾(25∶75,用磷酸调p H至3.5);流速为1.0 ml·min^(-1);检测波长为250 nm;柱温为30℃;进样量为20μl。结果:安乃近质量浓度在50.11~601.36μg·ml^(-1)(r=0.999 9)内,葛根素质量浓度在2.64~31.74μg·ml^(-1)(r=0.999 8)内与峰面积呈良好的线性关系。安乃近和葛根素的平均回收率分别为99.1%(RSD=0.7%,n=9)和99.9%(RSD=0.6%,n=9)。结论:该法简便准确、重复性好,可用于该制剂的质量控制。

Objective ： To establish an HPLC method for the determination of two components in compound artemisinin analgin tab- lets. Methods： A Waters Symmetry C,s column （250 mm ×4.6 mm,5 μm） was used with methanol-0.02 mol . L-1KH2PO4solution （25 ： 75, adjusting pH to 3.5 with phosphoric acid ） as the mobile phase. The flow rate was 1.0 ml . min-1 and the detection wave- length was 250 nm. The column temperature was 30℃ and the injection volume was 20 μl. Results： There was a good linear relation- ship when the content of analgin and puerarin was within the range of 50.11-601.36 μg . ml - 1 （ r = 0. 999 9） and 2.64-31.74 μg . ml - 1（ r = 0. 999 8 ）, respectively. The average recovery was 99.1% （RSD = 0.7%, n = 9） and 99.9% （ RSD = 0.6%, n = 9 ）, respec- tively. Conclusion： The method is simple and accurate with good reproducibility, which can be used to control the quality of the prepa- ration.