雷公藤口服液中雷公藤甲素、总二萜内酯和总生物碱的含量测定

Determination of Triptolide,Total Diterpenoids and Total Alkaloids in Leigongteng Oral Solution

目的:建立雷公藤口服液中雷公藤甲素、总二萜内酯以及总生物碱的含量测定方法,为质量控制提供依据。方法:采用高效液相色谱(HPLC)法,Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速1.0 ml·min^(-1),柱温40℃,检测波长218 nm,测定雷公藤口服液中雷公藤甲素的含量。采用紫外可见分光光度法,分别以雷公藤甲素、雷公藤次碱为对照,测定雷公藤总二萜内酯、总生物碱的含量。结果:雷公藤甲素、总二萜内酯(以雷公藤甲素计)和总生物碱(以雷公藤次碱计)的线性关系良好(r≥0.999 8),平均加样回收率分别为91.96%、90.73%、99.18%,RSD均小于3%。结论:本研究所建立的方法稳定可靠,重复性良好,可用于雷公藤口服液的质量控制。

Objective： To establish a method for the content determination of triptolide, total diterpenoids and total alkaloids in Leigongteng oral solution to provide basis for the quality control. Methods： An HPLC analysis was used to detect the content of triptol- ide, which was carried out on an Eclipse XDB-C18column （ 150mm×4.6mm,5μm） with the mobile phase consisting of aeetonitrile- water with gradient elntion. The flow rate was maintained at 1.0 ml. min -1, the column temperature was kept at 40℃ and the detec- tion wavelength was set at 218 nm. Using triptolide and wilforine as the contrast, the total diterpenoids and total alkaloids were deter- mined by UV-Vis speetrophotometry. Results ： There were good linear relationship in the determination of triptolide, total diterpenoids （eaculated as triptolide） and total alkaloids （caculated as wilforine） （r ≥0. 999 8 ）, the average recovery were 91.96%, 90.56%, 99.18%, and the RSD were less than 3%. Conclusion： The method is with good reproducibility and stability, which can be used for the quality control of Leigongteng oral solution.