基于COI和D-Loop序列豫北虾虎鱼分子系统分析

Molecular Phylogenetic Analysis of Gobies in the North of Henan Based on COI and D-Loop Sequences

虾虎鱼在河南省北部广泛分布,但其系统分类和多样性未有报道.从豫北原阳黄寺、安阳小南海、淇县淇河、长垣孙东闸、林州弓上水库等地采集虾虎鱼样本共计23尾,成都岷江样本6尾,分别扩增了线粒体COI基因和D-Loop序列.联合GenBank下载虾虎鱼的COI序列和D-Loop序列,构建了虾虎鱼的系统进化树.结果显示,采自黄寺、小南海、淇县、岷江地区的子陵吻虾虎鱼(Rhinogobius giurinus)聚为一个分支,而采自孙东闸和弓上水库的褐吻虾虎鱼(Rhinogobius brunneus)聚为一个单系群;这两种虾虎鱼遗传分化主要来自群体间.系统发育结果支持子陵吻虾虎鱼的韩国群体与中国群体构成姐妹群关系,中国不同地理种群间则相互交叉.

Gobies are widely distributed in the northern regions of Henan,phylogenetic classify and genetic diversity of which haven＇t been reported.Based on the sequences of COI and D-Loop of the obtained 23 specimen from the northern regions of Henan（Huangsi village of Yuanyang county,Qihe River of Qi County,Sundongzha of Changyuan county,Xiaonanhai Reservoir of Anyang city,Gongshang Reservoir of LinZhou city）and 6specimen from Min River（ChengDu,Sichuan province）,and the sequences download from GeneBank,phylogenetics tree was constructed.The results indicate that the gobies from Huangsi,Xiaonanhai,Qihe,Minjiang are Rhinogobius giurinus,which clades a monophyletic group,and the gobies from Sundong and Gongshang reservoir are Rhinogobius brunneus,which clades into a monophyletic group.The main sequence differentiation is between the two species.The phylogenetic tree indicates that R.giurinus from South Korea,constitutes a sister group with the population from China,and the different geographic populations from China are shown to be nested.