不同剂量瘦素预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用

Protective Effects of Different Doses of Leptin Pretreatment on the Myocardial Ischemia Reperfusion Injury in Diabetic Rats

目的:探讨不同剂量瘦素预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用。方法:将72只大鼠随机分为对照组、假手术组、心肌梗死组以及大、中、小剂量瘦素预处理组。对照组给予普通饲料喂养,其余各组均给予高糖高脂喂养。1个月后,采大鼠静脉血,检测其血清FBG、FTNS、瘦素、HOMA、TNF-α、MDA及血脂水平。结果:与对照组相比,其余各组FBG、FINS、瘦素水平及HOMA-IR均明显升高(P<0.05);心肌梗死组及大、中、小剂量瘦素预处理组FBG及瘦素水平与假手术组相比均明显升高,心肌梗死组、高剂量瘦素预处理组FINS及HOMA-IR与之相比亦明显升高,小、中剂量瘦素预处理组FINS及HOMA-IR与之相比均明显降低(P<0.05);小、中剂量瘦素预处理组FBG、FINS、瘦素水平及HOMA-IR与心肌梗死组相比均明显降低(P<0.05)。与对照组相比,其余各组大鼠TNF-α、MDA水平均明显升高(P<0.05);与假手术组相比,心肌梗死组、大、中、小剂量组大鼠TNF-α、MDA水平明显升高(P<0.05);与心肌梗死组相比,中、小剂量组大鼠TNF-α、MDA水平均明显降低(P<0.05)。与对照组相比,其余各组大鼠TG、TC、LDL-C水平均明显升高,HDL-C水平均明显降低(P<0.05);大、中、小剂量组HDL-C值与假手术组相比均明显升高,TG、TC、LDL-C值与之相比均明显降低(P<0.05);与心肌梗死组相比,小、中剂量组大鼠TG、TC、LDL-C水平均明显降低(P<0.05),HDL-C水平均明显升高(P<0.05)。结论:瘦素预处理能够保护糖尿病大鼠心肌缺血再灌注损伤,可能与其减轻炎症、氧化应激、血脂紊乱及胰岛素抵抗有关。

Objective： To discuss the protective effects of different doses of Leptin pretreatment on the Myocardial Ischemia Reperfusion Injury in Diabetic Rats. Methods： According to the random number table, 72 rats were divided into normal control group, sham operation group, myocardial infarction group and large, medium, small dose of Leptin pretreatment groups. Normal control group were fed with normal diet, the other groups were fed with high sugar and high fat feeding. After 1 months, all rats were abdominal vein blood, and then the FBG, FTNS, leptin, HOMA, TNF-α, MDA and serum lipid levels were measured in rats. Results： Compared with the control group, the FBG, fins, leptin level and HOMA-IR of other group were significantly rised （P〈0.05）; Compared with false-operation group, myocardial infarction group and large, medium, small dose of Leptin pretreatment group rats FBG and leptin levels were significantly increased, myocardial infarction group and high dose group rats, fins and HOMA-IR were significantly increased （P〈0.05）, low, middle dose of Leptin pretreatment group rats, fins and HOMA-IR were significantly lower （P〈0.05）; Compared with myocardial infarction group, the levels of FBG, FINS, leptin and HOMA-IR in small, medium dose of Leptin pretreatment group were significantly lower （P〈0.05）. Compared with the control group, the TNF-α, MDA levels of other groups were significantly increased （P〈0.05）; Compared with false-operation group, myocardial infarction group, myocardial infarction group and large, medium, small dose of Leptin pretreatment group rats TNF alpha, MDA level increased significantly （P〈0.05）; Compared with the myocardial infarction group, small dose of Leptin pretreatment group rats TNF alpha, MDA level were significantly lower （P〈0.05）. Compared with the control group, the TC, TG, LDL-C levels of other groups were significantly increased, HDL-C levels were significantly reduced （P〈0.05）; Compared with the sham operation group and large, medium, small dose ofLeptin pretreatment group rats HDL-C levels were elevated, and levels of TG, TC, LDL-C were significantly lower （P〈0.05）; compared with the myocardial infarction group, small dose of Leptin pretreatment group rats TG, TC, LDL-C levels were significantly lower （P〈0.05）, HDL-C levels were significantly increased （P〈0.05）. Conclusion： Leptin pretreatment can protect myocardial ischemia and reperfusion injury in diabetic rats, which may be related to the reduction of inflammation, oxidative stress, blood lipid disorder and insulin resistance.