miR-139-5p在小细胞肺癌组织中的表达及其临床意义

Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance

目的：探讨miR-139-5p在小细胞肺癌组织中的表达及其临床意义,阐明miR-139-5p在小细胞肺癌发生发展中的作用。方法：采用细胞生长、凋亡及周期实验检测miR-139-5p的生物学功能;实时荧光定量PCR法检测50例小细胞肺癌患者癌组织及癌旁正常组织中miR-139-5p的表达,结合临床资料分析其在临床中的作用。结果：miR-139-5p在小细胞肺癌组织中的表达水平低于正常肺组织（P〈0.01）;转染miR-139-5p mimics后能够明显上调细胞中miR-139-5p的表达,差异有统计学意义（P〈0.01）。与对照组比较,上调miR-139-5p的表达后细胞的增殖能力降低（P〈0.01）,G1期细胞明显增多（P〈0.05）,细胞周期发生G_0/G_1期阻滞。单因素分析,miR-139-5p的表达与患者的性别和年龄无关（P〉0.05）,miR-139-5p在局限期（LD）患者中的表达较广泛期（ED）患者低,两者的miR-139-5p表达率比较差异有统计学意义（χ~2=10.546,P=0.001）;miR-139-5p在耐药患者中的表达较化疗敏感患者增高（χ~2=7.025,P=0.008）。miR-139-5p在存活患者中的表达较死亡患者降低（χ~2=7.697,P=0.006）;Cox回归分析,疾病分期和miR-139-5p的表达可作为小细胞肺癌独立的预后因子。结论：miR-139-5p通过抑制细胞增殖、促进细胞凋亡及诱导细胞周期阻滞参与调节小细胞肺癌发生发展,可作为评估小细胞肺癌患者临床预后的靶基因。

Objective：To explore the expression of miR-139-5p in small cell lung cancer （SCLC）tissue and its clinical significance, and to clarify the role of miR-139-5p in the occurrence and development of SCLC. Methods：The biological function of miR-139-5p was examined by cell growth,apoptosis and cell cycle analysis. The expressions of miR-139-5p in 50 cases of cancer tissue and paracarcinoma normal tissue were examined by QRT-PCR.Combined with the clinical data,the role of miR-139-5p in clinic was anzlyzed.Results：The expression level of miR-139-5p in SCLC tumor tissue was lower than that in normal lung tissue （P 〈0.01）.The expression level of miR-139-5p in the cells was un-regulated significantly after transferred miR-139-5p mimics （P 〈0.01）.Compared＆amp;nbsp;with control group,the proliferation abilitiy of cells was reduced after up-regulating the expression of miR-139-5p （P 〈 0.01）,the number of cells at G1 phase was increased （P 〈 0.05）,and the cells were arrested at G0/G1 phase.The miR-139-5p expression was not associated with gender and age （P 〉 0.05）.The expression level of miR-139-5p in the patient at LD stage was lower than that of the patients at ED stage （P 〈0.01）.The expression level of miR-139-5p in the resistant patients was higher than that of the patients sensitive to chemotherappy （P 〈0.01）.The expression level of miR-139-5p in the survival patients was lower than that in the death patients （P 〈0.01）.Cox regression analysis indicated that miR-139-5p expression and disease stage were the independent prognostic factors for SCLC.Conclusion：miR-139-5p in participates in the occurrence and development of SCLC by inhibiting the cell proliferation,promoting apoptosis and inducing the cell cycle arrest;it may be used as a target gene to evaluate the prognosis of SCLC patients.