急性冠脉综合征患者内皮细胞微粒与CD4^+ CD25^+ Foxp3^+调节性T细胞的关系

Relationship between endothelial microparticles and CD4^+ CD25^+ Foxp3^+ regulatory T cells in patients with acute coronary syndrome

目的:探讨急性冠脉综合征(ACS)患者外周血内皮细胞微粒(EMPs)水平与CD4^+CD25^+Foxp3^+调节性T细胞(Treg)及其细胞因子水平的关系,阐明EMPs通过影响Treg细胞分化和功能参与ACS发病过程的机制。方法:选取稳定性心绞痛患者23例(SAP组),ACS患者52例(ACS组);以冠状动脉造影检查正常的住院患者20例作为对照组。采用流式细胞术检测各组患者外周血EMPs水平和Treg细胞百分率,实时定量PCR法检测转录因子Foxp3mRNA表达,酶联免疫吸附法检测血浆TGF-β1水平。对EMPs与Treg细胞、Foxp3mRNA表达水平和TGF-β1水平进行直线相关分析。结果:与对照组和SAP组比较,ACS组患者外周血中EMPs水平明显升高(P<0.01),CD4^+CD25^+Foxp3^+Treg百分率、Foxp3 mRNA表达水平和血浆TGF-β1水平明显降低(P<0.01)。EMPs与CD4^+CD25^+Foxp3^+Treg、Foxp3mRNA表达水平和TGF-β1水平呈负相关关系(r=-0.452,P=0.001;r=-0.466,P=0.001;r=-0.555,P=0.000)。结论:EMPs可能通过调控CD4^+CD25^+Foxp3^+Treg的分化和功能,参与冠状动脉粥样硬化的发生发展及斑块不稳定的过程。

Objective：To explore the relationships between the endothelial microparticles （EMPs）in peripheral blood and CD4＋ CD25 ＋ Foxp3＋ regulatory T cells and their cytokine levels in the patients with acute coronary syndrome （ACS）,and to clarify the mechanism of EMPs in the pathogenesis of ACS by affecting the Treg cell differentiation and function.Methods：Twenty-three patients with stable angina （SAP）were allocated to SAP group,and 52 patients with ACS were allocated to ACS group.Twenty individuals with normal conventional coronary angiography results were recruited as control group.The levels of EMPs and the percents of CD4＋ CD25 ＋Foxp3＋ regulatory T cells in peripheral blood of the patients in various groups were measured by flow cytometry. The expressions of Foxp3 mRNA and the plasma levels of TGF-β1 were detected with Real-Time PCR and ELISA, respectively.Correlation analysis was performed between EMPs and regulatory T cells,Foxp3 mRNA expression＆amp;nbsp;level and TGF-β1 level.Results：Compared with control group and SAP group,the level of EMPs in peripheral blood of the patients in ACS group was increased significantly,but the percentage of regulatory T cells,the Foxp3 mRNA expression level and the plasma TGF-β1 level were decreased markedly （P 〈0.01）.The correlation analysis showed that there were significantly negative correlation between EMPs and regulatory T cells,Foxp3 mRNA expression level and TGF-β1 level （r = - 0.452,P = 0.001;r = - 0.466,P = 0.001;r = - 0.555,P = 0.000 ）. Conclusion：EMPs may involve in the process of occurrence and development of ACS and plaque instability by regulating the differentiation and function of CD4＋ CD25 ＋ Foxp3 ＋ regulatory T cells.