煤矿巷道中低浓度甲烷微生物降解效能实验研究

Experimental Research on the Degradation Efficiency of Low Concentration Methane in Coal Mine Tunnels by Microorganism

为了研究煤矿低浓度甲烷微生物降解效能,富集、纯化分离并初步鉴定出可以甲烷作为唯一碳源的高效降解甲烷的好氧型微生物。生理生化实验表明,该菌符合甲烷氧化菌的典型特征。自主设计了模拟巷道中低浓度甲烷微生物降解实验分析系统。实验结果表明,在风速为0.5～1.0 m/s,甲烷体积分数为1%～5%范围内,随着甲烷体积分数的增加,甲烷的降解效果越明显,然而风速越大越不利于甲烷的降解,经过50 min的降解,甲烷体积分数由最高的5%最低降至1.43%;二氧化碳体积分数可由初始的0.031%最大升高到0.075%。实验还发现消耗的甲烷和生成的二氧化碳并非符合1∶1的比例。

In order to study the degradation efficiency of low concentration methane in coal mine by microorganism,enrichment,purification and isolation and preliminary identification of microorganism with high degradation ability are conducted,in which methane is used as the sole carbon source; a kind of simulation tunnel low concentration methane degradation analysis and experiment system is designed. Experimental results show that： in the range of wind speed 0. 5 ～ 1 m / s,methane concentration 1% ～ 5%,with the increase of methane concentration,the degradation effect of methane is more obvious,however,the high wind speed is unfavorable to the degradation of methane and after degradation by 50 minutes,methane concentration from the maximum of 5% can be reduced to 1. 43%; the concentration of carbon dioxide can be increased from 0. 031% to 0. 075% and in the experiment we also find that the consumption of methane and increasing amount of carbon dioxide is not in accordance with the proportion of 1∶ 1.