391株结核分枝杆菌基因分型研究和耐药性分析

Analysis of genotyping and drug resistance of 391 strains of Mycobacterium tuberculosis

目的探讨寡核苷酸分型(Spoligotyping)用于安徽省结核分枝杆菌临床分离菌株的分型,以初步了解安徽省结核分枝杆菌的基因型特征。方法连续收集安徽省胸科医院391株结核分枝杆菌临床分离菌株,进行Spoligotyping,采用Bio Numerics软件进行聚类分析。结果北京家族占86.19%(337/391),非北京家族占13.81%(54/391),54株非北京家族基因型中,T家族36株(包括T1 22株、T2 10株、T3 5株、T4 1株),占9.72%;H(Haarlem)家族4株,占1.02%;另有14株(3.58%)为New Found型,北京家族菌株对一线药物总的耐药率为23.14%(78/337),非北京家族菌株为27.78%(15/54),经卡方检验两者间的差异无统计学意义(P>0.05)。结论安徽省结核分枝杆菌存在明显的基因多态性,主要流行型为北京家族,北京家族与耐药无明显相关性。

Objective To evaluate the application of Spoligotyping in the genotyping of clinical Mycobacterium tuberculosis isolates from Anhui,in order to understand the characteristics of the genotypes of Mycobacterium tuberculosis in Anhui. Methods A total of 391 clinical M. tuberculosis isolates were continuously collected in Anhui Province Chest Hospital for Spoligotyping and cluster analysis with Bio Numerics software. Results 337 isolates（ 86. 19%） belong to the Beijing genotype,while 54 isolates（ 13. 81%） were non- Beijing family. Among the non- Beijing family,36 isolates belong to the T family（ 9. 72%）,including22 from the T1 family,10 from the T2 family,5 from the T3 family,1 from the T4 family,and 4 isolates were from the Haarlem family（ 1. 02%）. Additionally,14（ 3. 58%） strains were New Found type. The resistance rate of Beijing family strains against the first- line drugs was 23. 14%（ 78 /337）,while that of non- Beijing family strains was 27. 78%（ 15 /54）. However,chi-square test indicted that the difference between both of them was not statistically significant（ P 0. 05）. Conclusion It is evident that significant genetic polymorphism exists among the clinical strains of M. tuberculosis. Beijing family genotypes were still the predominant genotype,which has no obvious association with the drug- resistance.