刀豆蛋白A对CD4^+CD25^+调节性T细胞CCR4和CCR6表达的影响被引量：3

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摘要目的探讨刀豆蛋白A(Con A)对CD4+CD25+调节性T细胞趋化因子受体4(CCR4)和CCR6表达的影响。方法采用密度梯度离心法分离1名健康志愿者的外周血单个核细胞,免疫磁珠阴性分选法富集CD4+T细胞。采用流式细胞术分选出CD4+CD25+调节性T细胞和CD4+CD25-T细胞,并分析其纯度及存活率。采用20μg/m L Con A对细胞进行刺激,流式细胞术检测两种细胞刺激0、24、48 h的CCR4和CCR6表达。结果流式细胞术分选CD4+CD25+调节性T细胞的纯度为97.8%,CD4+CD25-T细胞为90.1%,两种细胞存活率均>90%。随着Con A刺激时间延长,CD4+CD25+调节性T细胞和CD4+CD25-T细胞CCR4表达均呈升高趋势,CCR6表达均呈先升高后降低趋势;Con A刺激0、24、48 h,CD4+CD25+调节性T细胞CCR4、CCR6表达均高于CD4+CD25-T细胞(P均<0.01)。结论 CD4+CD25+调节性T细胞CCR4、CCR6表达均升高,经Con A刺激后CCR4表达呈时间依赖性。

作者杨永红罗静袁军王树辉

机构地区贵州省骨科医院贵州省人民医院

出处《山东医药》 CAS 北大核心 2016年第32期38-40,共3页 Shandong Medical Journal

基金贵州省优秀科技人才省长专项基金资助项目[资黔省专字(2005)144号]

关键词调节性T细胞趋化因子受体4 趋化因子受体6 刀豆蛋白A T淋巴细胞

分类号 R557.3 [医药卫生—血液循环系统疾病]

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