蘘荷总黄酮的超声辅助提取工艺及其抗氧化活性研究

Ultrasonic-Assisted Extraction Process of Total Flavonoids from Zingiber mioga and Its Antioxidant Activity

目的利用响应面法确定蘘荷总黄酮的最佳超声辅助提取工艺,并探讨其抗氧化活性。方法在单因素实验的基础上,进行4因素3水平的Box-Behnken中心组合实验设计,利用响应面分析优化工艺条件,并对此工艺下的提取液进行抗氧化活性检测。结果在超声功率300 W时,蘘荷总黄酮最佳提取工艺条件为处理温度60℃、乙醇体积分数85.99%、液料比98.08 mL·g^(-1)、处理时间15 min,此工艺条件下蘘荷总黄酮得率为37.01%,与预测值35.49%的相对误差仅4.28%;1.038 7mg·mL^(-1)囊荷总黄酮与56.60μg·mL^(-1)Vc的还原力相当,一定浓度的囊荷总黄酮对·OH、DPPH自由基清除率达到73.07%、65.72%,对脂质体过氧化的抑制率达到49.94%。结论蘘荷总黄酮可作为一种新型抗氧化药物开发,其提取工艺可用响应面法进行回归分析和参数优化。

OBJECTIVE To determine the optimum ultrasonic-assisted extracting process of total flavonoids from Zingiber mioga by response surface methodology, and then study the antioxidant activity of the extract. METHODS On the basis of single factor ex- periments, a 4-factor, 3-level Box-Behnken center-united experiment was conducted. The Box-Behnken experiment analyzed and opti- mized the processing conditions by response surface methodology, then the antioxidant activity of the extract was determined. RESULTS The optimum conditions at ultrasonic power of 300 W were as follows ： treatment temperature 60 ℃ , ethanol concentration 85.99% , liquid-to-solid ratio 98.08 mL · g-1, treatment time 15 min. Under the optimum conditions, the extraction rate of the total flavonoids was 37.01% , with a bias of 4. 28% compared with the predicted value of 35.49% ; the reducing power of the total fla- vonoids from Zingiber mioga at the concentration of 1. 038 7 mg · mL-1 was equal to that of vitamin C of 56. 60 μg · mL-l ; the · OH radical scavenging ratio of the total flavonoids from Zingiber mioga was 73.07% , the DPPH radical scavenging ratio was 65.72%, and the inhibition rate of anti-lipid peroxidation was 49. 94%. CONCLUSION The total flavonoids from Zingiber mioga can be developed as a new type of antioxidant agents, and regression analysis and parameter optimization of the extracting process can be conducted by using response surface methodology.