MicroRNA-625在食管鳞癌中的表达及临床意义

Clinical significance of microrna-625 expression in esophageal squamous cell carcinoma

目的:探讨micro RNA-625(mi R-625)在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中的表达及其与临床参数的相关性,探究mi R-625对ESCC细胞系KYSE70迁移和增殖的影响。方法:实时荧光定量(real-time polymerase chain reaction,PCR)检测2014年2月至2015年4月郑州大学第一附属医院手术切除的86例ESCC及癌旁正常组织、ESCC细胞系和正常永生化食管上皮细胞系中mi R-625的表达,统计学分析其表达水平与ESCC患者临床病理参数及预后的相关性。Transwell实验检测mi R-625对细胞迁移能力的影响,细胞计数Kit-8(CCK-8)法检测mi R-625对细胞增殖的影响。结果:ESCC组织中mi R-625的表达明显低于癌旁正常组织(P<0.05),ESCC细胞系中mi R-625表达水平与正常永生化食管上皮细胞相比,显著下调(P<0.05)。mi R-625的表达与肿瘤直径、分化程度及淋巴结转移呈负相关(P<0.05)。随访数据提示mi R-625低表达组患者预后更差(P<0.05)。mi R-625能够抑制ESCC的迁移和增殖(P<0.05)。结论:mi R-625作为抑癌基因参与ESCC的发生发展,提示mi R-625可能作为一个ESCC治疗靶点和预后判断的分子标志物。

Objective： To analyze the correlation of miR-625 expression with clinicopathological characteristics in esophageal squa- mous cell carcinoma （ESCC） and to explore the effect of miR-625 on the migration and proliferation of ESCC cells. Methods： The expres- sion level of miR-625 was determined through real-time PCR in 86 paired human ESCC tissue specimens and tumor-adjacent normal esophageal tissue specimens, ESCC cell lines, and esophageal epithelial cell line. The associations of miR-625 expression with clinico- pathological characteristics and survival in ESCC patients were analyzed. Transwell and CCK-8 assays were performed to examine the effect of miR-625 expression on migration and proliferation of ESCC cells. Results： Compared with tumor-adjacent normal specimens, miR-625 was significantly downregulated in ESCC tissue specimens （P〈0.05）. MiR-625 expression was decreased in ESCC cell lines compared with human esophageal epithelial cell lines （P〈0.05）. Lower miR-625 expression was associated with poorer prognosis and survival. The migration and proliferation abilities of ESCC cells were inhibited by miR-625 overexpression （P〈0.05）. Conclusion： MiR-625 acts as a tumor suppressor gene in the development and progression of ESCC, suggesting that miR-625 may serve as an efficient prognosis biomarker and a potential therapeutic target for ESCC.