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窄谱中波紫外线照射对白癜风自体表皮移植的作用研究 被引量:9

Effects of narrow-band ultraviolet B radiation on the efficacy of autologous epidermal grafting for vitiligo
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摘要 目的:探讨自体表皮移植联合窄谱中波紫外线(NB-UVB)照射治疗白癜风的临床疗效及可能作用机制。方法30例白癜风患者分为单纯表皮移植组和表皮移植联合NB-UVB组。用酶联免疫吸附测定检测局部皮损组织液中白细胞介素18( IL-18)、肿瘤坏死因子α( TNF-α)以及可溶性细胞间黏附分子1(sICAM-1)水平,并进行疗效评估。结果治疗前,单纯表皮移植组和表皮移植联合NB-UVB组局部皮损组织液中IL-18分别为(0.985±0.305)μg/L和(1.008±0.399)μg/L;TNF-α分别为(1.132±0.313)μg/L、(1.147±0.474)μg/L;sICAM-1分别为(1.215±0.284)μg/L、(1.221±0.432)μg/L;水平明显高于健康对照组(均P〈0.01)。表皮移植联合NB-UVB组治疗后各细胞因子水平基本恢复正常,有效率(68.9%)明显高于单纯表皮移植组(43.8%),P〈0.05。结论白癜风患者局部皮损处存在免疫异常,NB-UVB照射可以通过调节机体免疫功能提高表皮移植的成功率。 Objective To evaluate the clinical efficacy of autologous epidermal grafting combined with narrow-band ultraviolet B(NB-UVB)radiation for the treatment of vitiligo, and to explore their possible action mechanisms. Methods Totally, 30 patients with vitiligo were randomly and equally divided into 2 groups:epidermal grafting group treated with autologous epidermal grafting alone, and combination group treated with both autologous epidermal grafting and NB-UVB radiation. Fifteen healthy volunteers served as the healthy control group. Enzyme-linked immunosorbent assay(ELISA)was performed to detect the levels of interleukin-18(IL-18), tumor necrosis factor-α(TNF-α)and soluble intercellular adhesion molecule-1 (sICAM-1) in interstitial fluid from the patients before and after the treatment, as well as from the healthy controls. Therapeutic effects were evaluated after 3-month treatment. Results Before the treatment, the epidermal grafting group and combination group both showed significantly increased levels of IL-18(0.985 ± 0.305μg/L, 1.008 ± 0.399μg/L, respectively), TNF-α(1.132 ± 0.313μg/L, 1.147 ± 0.474μg/L, respectively)and sICAM-1(1.215 ± 0.284μg/L, 1.221 ± 0.432μg/L, respectively)in interstitial fluid compared with the healthy control group(all P〈0.01). The levels of the three cytokines in the combination group almost all returned to normal after the 3-month treatment. In addition, the combination group showed a significantly higher response rate compared with the epidermal grafting group(68.9%vs. 43.8%, P〈0.05). Conclusion Immune abnormalities exist in local lesions of patients with vitiligo, and NB-UVB radiation can increase the success rate of epidermal grafting likely by regulating the body′s immune function.
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2016年第10期728-731,共4页 Chinese Journal of Dermatology
关键词 白癜风 紫外线 白细胞介素18 肿瘤坏死因子Α 细胞黏附分子 自体表皮移植 Vitiligo Ultraviolet rays Interleukin-18 Tumor necrosis factor-alpha Cell adhesion molecules Autologous epidermai grafting
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参考文献8

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二级参考文献18

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