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毛白杨不同组织器官稳定表达看家基因的筛选 被引量：4

Validation of reference genes for gene expression analysis in different tissues of Populus tomentosa
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摘要 以毛白杨(Populus tomentosa)‘BJHR01’的不同组织器官为材料,运用实时荧光定量PCR(qRT-PCR)技术及内参基因表达稳定性分析软件Best Keeper、Norm Finder和geNorm分析了8个看家基因在毛白杨不同组织器官中的表达稳定性。所选看家基因包括细胞骨架蛋白肌动蛋白基因(actin)、微管蛋白基因(TUB)、18S核糖体RNA基因(18S rRNA)、翻译延伸因子(EF-1 beta)、翻译起始因子(eIF 5AV)、组蛋白编码基因(H3)、核糖体蛋白基因(RP)和泛素蛋白基因(UBQ),结果发现eIF 5AV、actin和UBQ三个内参基因在毛白杨不同组织器官中表达水平稳定,适合在定量PCR分析中作为内参基因;TUB的表达稳定性最差,不适宜作为毛白杨不同组织器官基因表达研究的内参基因。在毛白杨不同组织器官基因表达水平研究中,可选用eIF 5AV、actin和UBQ三个内参基因的几何平均数作为参照标准,实验数据的分析将更为准确。 Eight candidate reference genes were evaluated for the expression stability in Populus tomentosa tissues, including meristem tip, mature leave, young root, xylem and phloem. Analyses by ge Norm, Norm Finder and Best Keeper revealed that eukaryotic translation initiation factor 5AV（eIF 5AV）, actin and ubiquitin（UBQ） were the most stable genes in different tissues, and the combination of the three reference genes can give accurate and reliable normalization of quantitative real-time PCR（qRT-PCR） analysis for target gene expression in P. tomentosa tissues.
作者 王瑛 陈亚娟 丁莉萍 魏建华 王宏芝
机构地区 北京市农林科学院北京农业生物技术研究中心 首都师范大学
出处 《植物生理学报》 CAS CSCD 北大核心 2016年第8期1312-1320,共9页 Plant Physiology Journal
基金 国家高技术研究发展计划("863"计划)项目(2013AA-102702) 国家重点基础研究发展计划("973"计划)项目(2012CB114501)~~
关键词 实时荧光定量PCR 内参基因 基因表达水平 毛白杨 quantitative real-time PCR reference genes gene expression level Populus tomentosa
分类号 S792.117 [农业科学—林木遗传育种]
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参考文献28

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二级参考文献38

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植物生理学报
2016年 第8期

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