单面针茎不同极性部位抗菌及对MCF-7细胞抑制活性的研究

Antibacterial and Inhibitory Effects of Different Extracts from Stem of Zanthoxylum dissitum Hemsl on MCF-7 Cell

研究单面针茎不同极性部位的抗菌及抗肿瘤活性。本实验通过微孔比浊法比较了单面针茎各极性部位对3种革兰氏阴性菌:大肠杆菌、铜绿假单胞菌、幽门螺杆菌,4种革兰氏阳性菌:表皮葡萄球菌、枯草芽孢杆菌、藤黄微球菌、金黄色葡萄球菌共7种细菌进行抗菌能力评价;以MTT法测定了各极性部位对乳腺癌细胞MCF-7的生长抑制活性。实验结果表明,单面针茎各极性部位对本实验所有菌株,尤其是革兰氏阳性菌均有较强的抑制作用,其中以氯仿部分的抗菌效果最佳;本实验所有样品对MCF-7细胞均有较好的抑制作用,同样氯仿浸膏的抑制活性最强,对实验细胞株的半数抑制浓度IC50为2.35±0.97×10-1mg/m L,正丁醇浸膏部分的IC50值分别为3.19±0.54×10-1mg/m L,然而乙酸乙酯浸膏部分则相对最弱,其IC50值为6.47±0.94×10-1mg/m L。本实验结果揭示有望从氯仿、正丁醇浸膏中分离得到抗菌、抗乳腺癌活性较强的单体化合物,为单面针的后续开发提供了科学的依据。

The aim of this study was to investigate the antibacterial and inhibitory activities of the different extracts of Zanthoxylum dissitum Hemsl Stem on the proliferation of MCF-7 cells. All the extracts were evaluated for their antibacterial activities against three gram-negative bacteria： Escherichia coli,Pseudomonas aeruginosa,Helicobacter pylori and four gram-positive bacteria： Staphylococcus epidermidis,Bacillus subtilis,Micrococcus luteus,Staphylococcus aureus using microporous turbidimetry method. In addition,their cytotoxic activities were measured against breast cancer cells MCF-7 by MTT assay. The results showed that all the extracts displayed great inhibition effects on the tested bacteria,especially those gram-positive bacterial,the chloroform part showed the highest activity,notably; All of the parts showed significant inhibitory effects on the proliferation of MCF-7 cells,among which,the chloroform part exhibited highese antineoplastic activities with IC50 of 2. 35 ± 0. 97 × 10-1mg / m L. Moreover,the IC50 of n-butanol part was 3. 19 ± 0. 54 × 10-1mg / m L.However,the ethyl acetate extract was weakest with IC50 of 6. 47 ± 0. 94 × 10-1mg / m L. These results suggested that the chloroform and n-butanol extract possibly contained compounds with significantly antibacterial and antitumor activities.This research provided a scientific basis for the development of Z. dissitum.