六种中草药提取物对两株口腔致病菌的体外抗菌活性

Antibacterial Activity of Six Herbal Extract on Two Oral Pathogens in vitro

采用二倍稀释法,以中草药提取物中的总黄酮、总有机酸和总皂苷等主要活性成分为定量标准,测定艾叶、侧柏叶、金银花、蒲黄、三七、仙鹤草提取物对变异链球菌和牙龈卟啉单胞菌的最低抑菌浓度(MIC)与最低杀菌浓度(MBC);并用大孔吸附树脂进行富集和除杂,探讨其可能的抗菌成分。结果发现金银花提取物对两株菌具有较好的抗菌效果,且总黄酮和有机酸是其主要的抗菌成分;三七提取物纯化后总皂苷浓度增大了1.7倍,对牙龈卟啉单胞菌由无抑菌变成抑菌作用;其它4种草药提取物纯化前后抗菌效果无明显改变。因此金银花总黄酮和总有机酸可认为是其主要的抗菌成分;三七总皂苷很有可能是抑制牙龈卟啉单胞菌生长的物质。

In this study,double dilution method was used to test the antibacterial activity of the main active ingredient of herbal medicine,such as total flavonoids,organic acids and total saponins,which were extracted from Artemisia argyi,Platycladus orientalis,Lonicera japonica,Pollen typhae,Panax notoginseng and Agrimonia pilosa against Streptococcus mutans and Porphyromonas gingivalis. The component were further enriched and purified with macroporous adsorption resin to find their possible antibacterial ingredients,respectively. The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts were tested. The experimental results showed that L. japonica extracts containing total flavonoids and organic acid had a good antibacterial effect on two strains; After purification,the total saponins concentration of P. notoginseng extracts increased 1. 7 times,and its inhibitory effect on P. gingivalis appeared; The antibacterial effects of the other 4 herbal extracts were not significantly changed before and after purification by macroporous adsorption resin. Thus,it was concluded that the total flavonoids and total organic acids of L. japonica were possibly the main antibacterial components. The total saponins of P. notoginseng were probably the substances which inhibited the growth of P. gingivalis.