2015年～2016年江苏盐城地区市场活禽与野生鸟类中J亚型禽白血病分子病原学调查

Molecular epidemiology of subgroup J avian leukosis virus from poultry markets and wild birds during 2015 to 2016

为调查江苏省盐城地区市场活禽与野生鸟类中J亚型禽白血病病毒（ALV-J）的感染情况，本研究于2015年～2016年采集市场活禽粪便样品636份、野生鸟类粪便样品826份，提取RNA后利用荧光定量PCR方法检测ALV-J；利用普通PCR扩增阳性样品gp85基因，并进行克隆测序。荧光定量PCR结果显示，636份活禽粪便样品中有7份为阳性、826份野生鸟类粪便样品为阴性。PCR扩增、克隆测序得到2个当地土鸡ALV-J分离株的gp85基因，与国内外ALV-J参考病毒株同源性为87.6 %～95.3 %，并且2个样品在进化树的不同分支。本研究结果显示，市场活禽存在ALV-J感染，野生鸟类并未发现ALV-J，表明加强活禽流通过程中禽白血病的防控工作势在必行，同时需继续加强对野生鸟类中ALV-J的监测。

To investigate the infection status of subgroup J of avian leukosis virus （ALV-J） in live poultry markets and wild bird flocks in Yancheng, Jiangsu province, a total of 826 fecal samples from wild birds and 636 fecal samples from live poultry markets were collected and subjected to ALV-J detection by real-time PCR. The results shown no any positive sample was detected in the samples from wild birds, and only 7 positive samples were detected from live poultry markets. In addition, the gp85 gene of ALV-J were amplified from the 2 positive samples by PCR and sequenced for further analysis. The sequence analysis revealed that 2 positive gp85 gene （921 bp） of the ALV-J belonged to different genetic branch, The homology analysis showed 87.6% to 95.3% homology compared with domestic and international ALV-J reference strains. In conclusion, ALV-J infection existed in live poultry markets, more attention should be paid in preventing and controlling the transmission of ALV-J among wild birds.