泻肝凉血解毒方对豚鼠银屑病样皮损TNF-α的影响

Effects of “Xiegan Liangxue Jiedu Decoction”on TNF-α in psoriatic guinea pigs

目的探讨泻肝凉血解毒方治疗银屑病的作用机制。方法将豚鼠随机分为空白组、模型组、泻肝凉血解毒方组和阿维A胶囊组。采用普萘洛尔乳剂外涂豚鼠耳背皮肤复制银屑病模型。各组给予相应处理,疗程4周。观察豚鼠一般情况、局部皮损病理变化、皮损组织和血清肿瘤坏死因子(TNF-α)表达变化。结果 1模型组豚鼠耳部皮肤呈典型的银屑病样病理改变,泻肝凉血解毒方组和阿维A胶囊组皮损组织病理明显改善;2与空白组比较,模型组皮损组织和血清TNF-α水平升高(P<0.05);与模型组比较,泻肝凉血解毒方组和阿维A胶囊组皮损组织和血清TNF-α水平降低(P<0.05);但两治疗组之间TNF-α水平无显著性差异(P>0.05)。结论泻肝凉血解毒方治疗银屑病的机制可能与降低局部组织和血清TNF-α水平有关。

Objective To study the therapeutic mechanisms of ＂Xiegan Liangxue Jiedu Decoction＂ in the treatment of psoriasis. Methods The guinea pigs were randomly divided into the control group, model group, ＂Xiegan Liangxue Jiedu Decoction＂ group （ herb group） and aeitretin group. Popranolol emulsion was applled on ears back of guinea pigs to induce the psoriasis-like lesions. Each group was given corresponding treatment for 4 weeks. The general situation of guinea pigs, pathological changes of skin lesions, and the TNF-α expressions in the local skin and blood were observed. Results （1）The ear skin of the guinea pigs in the model group showed typical psoriatic lesions, which were obviously alleviated in herb group and acitretin group; （2）Compared with the control group, the levels of TNF-a in the blood and local lesions were increased in the model group （P 〈 0.05 ） ; compared with the model group, the levels of TNF-α in the blood and local lesions were decreased in the herb and acitretin groups （P 〈 0.05 ） ; while there was no significant difference in the level of TNF-α between the herb and acitretin groups （ P 〉 0.05 ）. Conclusion ＂Xiegan Liangxue Jiedu Decoction＂ acts to treat psoriasis by reducing TNF-α in the blood and local lesions.