抗流感病毒天然化合物体外筛选模型的建立及应用

Establishment and application of screen model for natural compounds with anti influenza virus activity in vitro

目的建立抗流感病毒天然化合物体外筛选模型。方法以扎那米韦为阳性药物,运用酶联免疫吸附实验(ELISA)和三磷酸腺苷荧光检测(ATPlite)技术,建立基于细胞培养的天然化合物抗流感病毒体外筛选模型,测定205种天然化合物的细胞毒性及其抗流感病毒活性。结果成功构建抗流感病毒天然化合物细胞体外筛选模型,以扎那米韦验证,流感病毒被有效抑制,对A型流感病毒的IC50为0.002 8μmol/L,对B型流感病毒的IC50为0.057μmol/L。205种天然提取化合物中,有143种无显著细胞毒性,应用体外模型筛选,未检出有抗流感活性的化合物。结论成功建立基于ELISA法和ATP荧光法的体外抗流感天然化合物细胞筛选模型,可用于抗流感药物大规模筛选。

Objective To establish a cell-based model to screen nature compounds with anti influenza virus activities invitro. Methods Zanamivir （Relenza） was used as positive control for evaluation of established model based on ELISA andATPlite fluorescence detection. A total of 205 natural compounds were tested for cytotoxicity and anti influenza virus activi-ty. Results Screen model was established and confirmed by positive control zanamivir, which inhibited influenza virus in themodel. The IC50of zanamivir against influenza A and B virus were 0. 002 8 /lmol/L, 0. 057 /lmol/L, respectively. Among 205natural compounds, 143 were confirmed without significant cytotoxicity; none of 143 compounds were positive for anti influen-za virus activities by the model. Conclusion The in vitro secreen model based on ELISA and ATP fluorescence detection issuccessfully established ,which can be used for high throughput screen for compounds with anti influenza virus activities.