甲单宁片对小鼠免疫力功能的影响研究

Study on immunity enhancing function of epigallocatechin gallate on mice

目的探讨甲单宁片对小鼠免疫功能的影响。方法 200只雌性ICR小鼠随机分为4组,即低、中、高剂量组和溶剂对照组。连续灌胃30d,采用二硝基氟苯诱导小鼠迟发性变态反应(耳肿胀法)、ConA诱导的小鼠脾淋巴细胞转化实验(噻唑蓝MTT法)进行细胞免疫功能测定;以抗体生成细胞检测(琼脂糖平板法)、血清溶血素的测定(半数溶血值HC50测定法)进行体液免疫功能测定;以小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验(滴片法)进行单核-巨噬细胞功能测定;以乳酸脱氢酶测定法进行NK细胞活性测定。结果以甲单宁片灌胃30d,小鼠细胞免疫功能、体液免疫功能、单核-巨噬细胞功能、NK细胞活性测定实验结果均为阳性;各剂量组小鼠体重、脏器/体重与对照组差异均无统计学意义(P值均>0.05)。结论实验条件下,甲单宁片可明显增强小鼠免疫力功能。

Objective To study effect of epigallocatechin gallate on immune function in mice. Methods A total o f 200 fe-male ICR mice were randomly divided into 4 groups, which were low, medium and high dose groups and solvent control group.Mice were subjected to intragastric administration for 30 d. The cellular immunity was evaluated by response of delayed hyper-sensitivity and lymphocyte proliferation of mouse spleen induced by ConA; humoral immunity was analyzed by detection of an-tibody producing cells （hemolytic plaque method） and serum hemolysin determination （HC50 assay） mononuclear macrophagecell activity was assessed by mice carbon particle clearance assay and peritoneal macrophage phagocytosis of chicken red bloodcell test NK cell activity was determined by lactate dehydrogenase assay. Results After 30 d of intragastric administration,significant differences were observed for mice cellular immune function humoral, immune function, mononuclear macrophagecell activity and NK cell activity. Conclusion Epigallocatechin gallate tablets can promote mice immune functions under experi-mental conditions.