餐饮垃圾制备乙醇的同步糖化发酵研究

Simultaneous saccharification and fermentation from food waste to ethanol

为建立一种针对餐饮垃圾的同步糖化发酵技术,进一步提高乙醇的产率,以期早日实现工业化,通过将单酶以两两组合或三个混合的方式筛选最优酶组合,结果显示终酶活力120 U/g的糖化酶与300 U/g的异淀粉酶之间的协同作用最好,葡萄糖产量达130.29 g/L。利用酿酒酵母CICC 1346、终酶活力为120 U/g的糖化酶和300 U/g的异淀粉酶对餐饮垃圾进行了同步糖化发酵(SSF)和分步糖化发酵(SHF),结果显示SSF和SHF的乙醇产量分别为49.48 g/L和45.89 g/L,糖醇转化率分别为91.7%和77.1%。SSF与SHF相比存在操作简单、发酵时间短和乙醇产率高的优势,已具备了工业化应用的价值。

The purpose of study is to establish a method to improve the ethanol output by saccharification and fermentation of food waste in an order to realize the industrialization in the future. By the way of combining two or three kinds of enzymes to obtain an optimization, it was shown that positive synergism effect was observed between glucoamylase （120 U/g） and isoamylase （300 U/g）, and the glucose concentration in the hydrolysate reached 130. 29 g/L. Simultaneous saccharification and fermentation （SSF） and separate hydrolysis and fermentation （SHF） of food waste have been conducted using Saccharomyces cerevisiae CICC 1346 and 120 U/g of glucoamylase and 300 U/g of isoamylase. The yields of ethanol in SSF and SHF were 49. 48 and 45. 89 g/L, and the conversion rates from glucose to ethanol were 91.7% and 77.1%, respectively. Compared to the SHF process, the SSF one was easier to operate and showed shorter fermentation period but higher ethanol production rate, which makes it more suitable for industrial application.