奥硝唑唾液浓度与血药浓度的相关性分析

Analysis of the Correlation between Ornidazole Salivary Concentration and Plasma Concentration

目的：探讨健康受试者奥硝唑（ONZ）唾液浓度与血药浓度的相关性,为临床治疗药物监测提供参考。方法：选择健康受试者24名,口服ONZ胶囊1.00 g后,分别于给药后的0.25、0.5、1.5、5.5、10.5、24.5、43.5 h采集静脉血和唾液,采用高效液相色谱法分别测定ONZ在血浆和唾液中的质量浓度,比较两者的相关性。结果：24名健康受试者血浆和唾液中ONZ质量浓度峰值均出现在给药后1.5 h,其峰值分别为（0.96±0.15）和（0.93±0.15）μg/ml;各时间点ONZ唾液浓度虽低于血药浓度,但差异均无统计学意义（P〉0.05）;唾液浓度与血药浓度的回归方程为c唾=1.176 5c血-0.199 4（r=0.990 1）,其平均质量浓度的比值（S/P）为（0.91±0.06）,两者呈正相关（r为0.632~0.970,P〈0.05）。结论：ONZ在健康受试者唾液中的质量浓度与血药浓度显著正相关,可应用唾液进行治疗药物监测。

OBJECTIVE： To explore the correlation between omidazole （ONZ） salivary concentration and plasma concentrations in healthy subjects, and to provide reference for clinical therapeutic drug monitoring. METHODS： 24 healthy volunteers were selected. After oral administration of ONZ capsules 1.00 g, their venous blood and saliva were collected at 0.25, 0.5, 1.5, 5.5, 10.5, 24.5 and 43.5 h after medication. HPLC method was used to determine the plasma and salivary concentrations of ONZ. The correlation between the two was analyzed. RESULTS： The peak values of plasma and salivary ONZ concentrations appeared immediately at 1.5 h after administration and the peak values were （0.96 ± 0.15）μg/ml and （0.93 ± 0.15） μg/ml; salivary concentration of ONZ was lower than plasma concentration at each time points, but there was no statistical significance （P〉0.05） ; the regression equation of salivary ONZ concentration and plasma concentration was csaliva= 1.176 5cplasma-0.199 4 （r=0.990 1）. The ratio of salivary concentration and plasma concentration of ONZ （S/P） was （0.91 ±0.06）, showing positive correlation （r=0.632-0.970, P〈0.05）. CONCLUSIONS： The salivary ONZ concentration is significantly correlated with plasma concentration in healthy people, so saliva can be used for therapeutic drug monitoring.