烧伤患者金黄色葡萄球菌分离株外毒素基因研究

Exotoxin genes of Staphylococcus aureus isolates in burn patients

目的了解烧伤患者金黄色葡萄球菌（SAU）分离株的各类外毒素基因携带状况,为临床SAU感染的治疗提供依据。方法收集2015年1-6月医院烧伤患者标本分离20株SAU,通过法国生物梅里埃公司VITEK-2compact全自动鉴定仪进行菌株鉴定,PCR方法分析外毒素基因（set1-26）和（set30-40）,采用Q型类进行聚类分析。结果 20株SAU经37种外毒素基因检测,只有1株未检出基因;其余19株均有阳性检出;外毒素基因中set2、set4、set16、set23、set37、set39等无阳性检出,其余阳性检出率在5.0%~95.0%;其中检出率〉50.0%有24种基因,〉75.0%有15种基因;外毒素基因检测结果具多样性,可分17种模式;聚类分析发现20株SAU可分为大、小两个家族。结论 20株SAU外毒素基因阳性率高,并可能与烧伤伤口脓疱疮和蜂窝织炎相关。

OBJECTIVE To investigate all kinds of exotoxin genes carrying status in Staphylococcus aureus isolates in burn patients,so as to provide the basis for the treatment for S.aureusinfection.METHODS S.aureus were isolated from the samples of our hospital burn patients from Jan.to Jun.2015,using Vitek-2compact automatic identification device to give strain identification,using PCR to analysis exotoxin genes set1-26 and set30-40,and using Q type to have cluster analysis.RESULTS After using 37 kinds of exotoxin genes to detect 20 strains of S.aureus,only 1strain was not detected any gene,and the remaining 19 strains had positive findings.Exotoxin genes only set2,set 4,set16,set23,set37,set39,etc had non positive findings,the positive rates for the rest were within 5.0% ~95.0%.There were 24 genes with positive rates more than 50%,15 genes more than 75%.The detection results of exotoxin genes had diversity with 17 patterns.Cluster analysis showed that the 20 strains of S.aureus could be classified into big and small families.CONCLUSIONThere are 20 strains of S.aureus exotoxin genes with high positive rates,which may be related to the impetigo in burn wound and cellulitis.