丹皮酚对Hacat细胞增殖及凋亡的影响

Effects of paeonol on the proliferation and apoptosis of Hacat cells

目的:明确丹皮酚对Hacat细胞增殖及凋亡的影响。方法:培养人角质形成细胞株Hacat,采用WST-8检测细胞丹皮酚对Hacat细胞增殖的影响;流式细胞仪检测丹皮酚对Hacat细胞细胞周期及凋亡的影响。结果:丹皮酚能有效抑制Hacat细胞的增殖,将Hacat细胞阻滞于G2/M期,同时丹皮酚可有促进Hacat细胞凋亡作用。结论:丹皮酚可抑制角质形成细胞的增殖,促进角质形成细胞的凋亡,可能是牡丹皮治疗银屑病的有效单体之一。

Objective： To identify the effects of paeonol on the proliferation and apoptosis of Hacat cells. Methods： Hacat cells were cultured by human cutin. The proliferation of Hacat cells was detected by WST-8. The cell cycle and apoptosis of Hacat cells were detected by flow cytometry. Results： Paeonol inhibited the proliferation of Hacat cells, and blocked the cell cycle in G2/M phase. Paeonol induced the apoptosis of Hacat cells. Conclusion： Paeonol can inhibit the proliferation and promote the apoptosis of Hacat cells, consequently which may be one of potential effective monomers of Cortex Moutan Radicis for treating psoriasis.