植物乳杆菌CLP0279发酵生产低温SOD培养基的优化

Optimization of fermentation medium for a cold-adapted superoxide dismutase(SOD) produced by Lactobacillus plantarum sp. CLP0279

【目的】提高植物乳杆菌CLP0279发酵生产低温超氧化物歧化酶(Superoxide dismutase,SOD)的能力。【方法】在单因素实验基础上,采用Plackett-Burman(PB)设计、Box-Behnken(BB)设计和响应面分析法(RSM),对发酵培养基进行优化。【结果】植物乳杆菌CLP0279产低温SOD最佳发酵培养基(g/L):玉米粉25.000,磷酸二氢钾2.600,磷酸氢二钾1.830,硫酸铜0.011,硫酸锌0.014。在最佳培养基条件下产酶活力达到194.82 U/m L,是优化前的1.36倍。【结论】通过响应面分析,对植物乳杆菌CLP0279发酵生产低温SOD的培养基进行优化,明显提高了产酶能力。确定了磷酸氢二钾、硫酸铜和硫酸铵为发酵培养基中影响酶活的3个关键因子。研究结果为SOD的发酵放大提供了依据。

[Objective] In order to increase the activity of a cold-adapted superoxide dismutase（SOD） from Lactobacillus plantarum sp. CLP0279. [Methods] Based on the single-factor experiment, the fermentation medium for the production of SOD was optimized using Plackett-Burman method, Box-Behnken design and response surface analysis. [Results] The optimal fermentation medium（g/L） was determined as follows： corn meal powder 25.000, KH2PO4 2.600, K2HPO4 1.830, Cu SO4·5H2O 0.011, ZnSO4·7H2O 0.014. Under these conditions, the maximum activity of SOD was 194.82 U/m L, which was 1.36 times comparing to that before optimization. [Conclusion] The activity of the cold-adapted SOD was obviously increased after optimization and three crucial factors（K2HPO4, Cu SO4·5H2O and ZnSO4·7H2O） were verified by the response surface analysis, which might provide a foundation for the scale-up culture in the future.