3种野生牛肝菌的菌塘土壤细菌多样性

Bacteria diversity of shiroes soil growing three species of wild Boletus

【目的】以远东疣柄牛肝菌(Leccinum extremiorientale)、美味牛肝菌(Boletus edulis)和小孢粉孢牛肝菌(Tylopilus microsporus)菌塘为研究对象,揭示其菌塘土壤细菌的菌群多样性。【方法】通过高通量测序方法,分析这3种野生牛肝菌菌塘土壤细菌的菌群结构及多样性。【结果】远东疣柄牛肝菌菌塘土样中的主要细菌类群为变形杆菌门(53.6%)、酸杆菌门(33.8%)、拟杆菌门(4.7%)、放线菌门(1.7%);美味牛肝菌中主要类群为酸杆菌门(40.7%)、变形杆菌门(38.1%)、拟杆菌门(10.3%)、放线菌门(3.1%);小孢粉孢牛肝菌中酸杆菌门(65.3%)、变形杆菌门(23.8%)、拟杆菌门(4.9%)为主要类群。细菌在属级分类水平上,远东疣柄牛肝菌菌塘主要属中69.8%为未知属,其余为伯克氏菌属Burkholderia(16.0%)、酸杆菌门中的Candidatus Solibacter(5.9%)和酸孢菌属Acidocella(3.3%)。美味牛肝菌中主要的属分布在未知类群(83.0%),此外,还有Candidatus Solibacter(8.0%),伯克氏菌属Burkholderia(2.1%)。小孢粉孢牛肝菌菌塘中,分别为未知类群(80.9%)和Candidatus Solibacter(12.6%)。【结论】3种牛肝菌菌塘土壤中细菌类群具有丰富的多样性,其中含有大量未知类群。

[Objective] In order to study the community diversity of shiroes soil, shiroes of Leccinum extremiorientale（Le）, Boletus edulis（Be） and Tylopilus microsporus（Tm） were investigated. [Methods] Bacterial diversity and community structure of shiroes of these wild Boletus were analyzed via high throughput sequencing. [Results] Major phyla of Le, Be and Tm were Proteobacteria（53.6%）, Acidobacteria（33.8%）, Bacteroidetes（4.7%）, Actinobacteria（1.7%）; Acidobacteria（40.7%）, Proteobacteria（38.1%）, Bacteroidetes（10.3%）; Acidobacteria（65.3%）, Proteobacteria（23.8%） and Bacteroidetes（4.9%）, respectively. The dominant genera of Le, Be and Tm were unclassified bacteria（69.8%）, Burkholderia（16.0%）, Candidatus Solibacter（5.9%）, Acidocella（3.3%）; unclassified bacteria（83.0%）, Candidatus Solibacter（8.0%）, Burkholderia（2.1%）; unclassified bacteria（80.9%）, Candidatus Solibacter（12.6%）, respectively. [Conclusion] The bacterial diversity is abundant in the three shiroes and there are a number of unclassified bacteria.