基于金针菇全基因组的赖氨酸合成途径关键酶分析

Analysis of genes related to lysine biosynthesis based on whole genome of Flammulina velutipes

【目的】以金针菇全基因组测序数据为基础研究其L-赖氨酸的从头合成途径及其关键基因。【方法】采用Illumina Hiseq2000和Roche454 FLX+两种方法完成金针菇单孢菌株Dan3的基因组测序,基于全基因组序列筛选金针菇中赖氨酸生物合成的关键基因,并分析这些基因编码的蛋白质基本理化性质;预测其亚细胞定位情况及蛋白的二级结构。【结果】金针菇Dan3全基因组序列长度为34.17 Mb,预测到8个参与α-氨基己二酸途径的关键基因,这些基因都含有多个内含子和外显子,二级结构主要由α-螺旋和无规则卷曲组成,有3个蛋白定位于线粒体。【结论】金针菇是通过α-氨基己二酸途径合成赖氨酸,基因组中预测到与该途径相关的几乎所有的酶。

[Objective] To analyze lysine biosynthetic pathway and related genes based on the whole genome of Flammulina velutipes. [Methods] Genome sequence of the monosporous strain Dan3 was sequenced through Illumina Hiseq2000 and Roche454 FLX＋. Key genes related to lysine biosynthesis pathway were screened based on the whole genome. Basic physical and chemical properties of these proteins were analyzed, and the subcellular localization and secondary structure were predicted. [Results] The sequenced reads were assembled and 34.17 Mb length genome sequence of Dan3 was obtained. The structure of 8 key genes involved in α-aminoadipate pathway was identified. Each gene was composed of a number of introns and exons, and the secondary structure was mainly composed of α-helix and irregular curl. Three of these proteins were localized to the mitochondria. [Conclusion] The de novo biosynthesis of lysine in F. velutipes was through the α-aminoadipate pathway, and almost all enzymes involved in this pathway were predicted in the whole genome of F. velutipes.