消毒愈肌膏对糖尿病足大鼠溃疡组织Akt-HIF-la信号通道的影响

Effects of Xiaodu Yuji Paste on Ulcer Diabetic Foot by Akt-HIF-la Signaling Channel

目的:观察消毒愈肌膏对糖尿病足大鼠溃疡组织HIF-la、VEGF等蛋白表达的影响。方法:将SD大鼠随机分为空白组、模型组、西药组、中药组和中西药组,每组30只。制作糖尿病足大鼠模型,模型组和空白组:常规饮食,在患处应用无菌盐水,无菌纱布包扎,每天换药1次。二甲双胍组:以浓度为1 mmol/L,10 m L灌胃,每天1次;在患处应用无菌盐水,无菌纱布包扎,每天换药1次。中药组:患处消毒愈肌膏,无菌纱布包扎,每天换药1次。中西药组:二甲双胍灌胃与患处消毒愈肌膏外敷相结合方式,观察3周。在不同时间段观察血糖的变化和创面的愈合程度用Western Blot技术检测大鼠溃疡组织HIF-la、VEGF等蛋白含量。结果:第7天,模型组、西药组、中药组和中西药组创面愈合率明显低于空白组,有显著性差异(P<0.05);西药组、中药组和中西药组创面愈合率明显高于模型组,有显著性差异(P<0.05);第14天和21天,模型组、西药组、中药组创面愈合率明显小于空白组,有显著性差异(P<0.05);西药组、中药组和中西药组创面愈合率明显高于模型组,有显著性差异(P<0.05);西药组、中药组和中西药组中各蛋白含量与模型组比较,有显著性差异(P<0.05)。结论:消毒愈肌膏治疗作用可能与促进Akt;HIF-la;VEGF;VEGFR2;SDF-la及CXCR4蛋白表达有关,从而促进新血管生成,加快创面愈合。

Objective ：To investigate the effects of Xiaodu Yuji Paste on HIF -la and VEGF of diabetic foot. Methods： A total of 120 rats of diabetic foot ulcer were randomly divided into four groups（ 30 each） to receive traditional Chinese medicine, Metformin and saline for 3 weeks. HIF - 1a and VEGF were extracted from the tissue near the wound by Western Blot and we observed the change of blood glucose and wound healing in the different time points. Results： The levels of wound healing of TCM group, Metformin group,TCM and Metformin group were higher than those in model group, but low- er than those in control group in the different time points. The levels of HIF - 1, AKT, VEGF, VEGFR2, SDF - 1 and CX- CR4 of TCM group, Mefformin group, TCM and Metformin group were higher than those in model group. Conclusion： These findings suggest that Xiaodu Yuji Paste can regulate the differential expression of some proteins,indirectly stimulating new vascular generation, in order to facilitate the wound healing.