复方黄芪当归汤对MS大鼠肾损害的干预作用及其机制研究

Compound Huangqi Danggui Decoction Intervening Renal Injury of MS Rats and Its Mechanism

目的:观察复方黄芪当归汤对MS大鼠肾损害的干预作用,论证其在诊治MS方面的药用价值。方法:制备大鼠代谢综合征模型,观察正常对照组、模型组、预防组、中药治疗组以及西药对照组,各组用药前后肾脏组织形态学病理改变,并观察对肾小管组织中MMPs及TIMPs的水平表达的影响。结果:(1)干预4周后,除预防组外各组大鼠的体质量均较正常对照组明显增加,差异显著(P<0.05);(2)与正常对照组相比,各组大鼠MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA相对表达量均显著增加,MMP-2/TIMP-2,MMP-9/TIMP-1比值均低于正常对照组(P<0.05);(3)各组大鼠MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA相对表达量均低于模型组,MMP-9/TIMP-1、MMP-2/TIMP-2比值均高于模型组,具有统计学差异(P<0.05)。结论:(1)复方黄芪当归汤预防和治疗组能减少MS模型大鼠肾组织MMP-2、TIMP-2、MMP-9、TIMP-1的mRNA表达,并升高MMP-2/TIMP-2、MMP-9/TIMP-1比值,改善MMPs/TIMPs平衡。(2)复方黄芪当归汤预防和治疗组能减轻MS模型大鼠的肾脏损伤。

Objective： To observe the effect of Compound Huangqi Danggui Decoction on renal injury of rats with MS and demonstrate the medicinal value in diagnosis and treatment of MS. Method： We established the rats model of MS and observed the morphological changes of renal tissue pathology as well as the expression levels of MMPs and TIMPs in renal tubular tissue before and after treatment in control group, model group, preventing group, traditional Chinese medicine group and western medicine group. Result ： （ 1 ） Four weeks after the intervention, all rats weights were significantly increased compared with those of other groups except preventing group and the difference was significant （ P 〈 0.05 ）. （2） The mRNA relative expression levels of MMP - 2, TIMP - 2, MMP - 9 and TIMP - 1 were increased significantly compared to those of the control group and the ratios of MMP - 2/TIMP - 2 and MMP - 9/TIMP - 1 were both lower than the control group＇ s （P 〈 0.05 ）. （ 3 ） The mRNA relative expression levels of MMP - 2, TIMP - 2, MMP - 9 and TIMP - 1 were lower in all groups compared to those of the model group and the ratios of MMP - 9/TIMP - 1 and MMP - 2/TIMP - 2 were higher than those of the model group and the difference was statistically significant （P 〈 O. 05）. Conclusion ： （ 1 ） The preventing and treatment groups by Compound Huangqi Danggui Decoction can reduce the mRNA expression levels of MMP - 2, TIMP - 2, MMP - 9 and TIMP - 1 and increase the ratios of MMP - 2/TIMP - 2 and MMP - 9/TIMP1 in renal tissues in MS rats （P 〈 0.05 ） as well as improve the balance of MMPs/TIMPs. （2） The preventing and treatment groups by Compound Huangqi Danggui Decoction can relieve the renal injury in MS rats.