四种产地蛇床子药材质量分析比较

Four Habitats Research and Analysis of the Quality of Fructus Cnidii

目的:通过不同实验方法,对湖北、安徽、浙江、山东四种产地蛇床子药材,进行品种和质量的实验分析,确定品质较优的蛇床子产地。方法:将四种产地蛇床子药材用95%乙醇回流提取,再经浓缩、离心、吸附获得供试品。以蛇床子中总香豆素为研究指标,采用性状分析、紫外分光光度法和薄层分析法测定四种产地蛇床子的总香豆素含量,综合分析进行比较。结果:紫外分光光度法测得山东、湖北、浙江、安徽四个产地的蛇床子,同一水平下的提取物在标准曲线上对应的浓度分别是0.1033 mg/m L、0.0181 mg/m L、0.0976 mg/m L、0.1096 mg/m L。薄层分析与标准品的对照显示中,四种产地的蛇床子以安徽的荧光点最大,山东次之,浙江产地的荧光点比湖北产地明显。结论:以香豆素为指标,四个产地中蛇床子的质量优质顺序为安徽>山东>浙江>湖北。

Objective： the experiment of variety and quality was carried out in different kinds of methods in Hubei,Anhui,Zhejiang,Shandong four areas of Fructus Cnidii,to determine the superior quality of Fructus Cnidii origin. Methods： Four herbs sampled with 95% ethanol extraction,concentration,centrifugal and absorption.Indicators of total coumarins in Fructus cnidii were used for research,by UV spectrophotometry method and thin- layer analysis method for the determination of total coumarins of Fructus cnidii from four habitats content analysis for comparison. Results： The UV spectrophotometer measured in Shandong,Hubei,Zhejiang,Anhui four areas with a concentration of extract of Fructus Cnidii,the concentration of the extract on the standard curve at the same level is 0. 1033 mg / m L,0. 0181 mg / m L,0. 0976 mg / m L,0. 1096 mg / m L. TLC analysis revealed that the control standard in four different places in Anhui＇s largest Osthol fluorescence point,Shandong times,Zhejiang origin of fluorescence better than Hubei origin significantly. Conclusion： Taking coumarin as indicators,four quality of osthole is in order for Anhui 〉Shandong〉 Zhejiang〉 Hubei.