IFI16 siRNA对干扰素α诱导的人脑血管外膜成纤维细胞增殖与凋亡的影响

Effect of IFI16 siRNA on Apoptosis and Proliferation of Interferon-α Induction of Human Brain Vascular Adventitial Fibroblasts

目的探讨干扰素诱导蛋白16(IFI16)siRNA对干扰素α(IFN-α)诱导的人脑血管外膜成纤维细胞(HBVAF)增殖与凋亡的影响。方法在HBVAF中转染IFI16 siRNA 48 h后,用2×106U/L IFN-α处理转染IFI16siRNA的细胞24 h,流式细胞术测定细胞周期及凋亡,real-time PCR和Western blot测定细胞中IFI16 mRNA和蛋白表达水平。结果转染IFI16 siRNA后,HBVAF中IFI16 mRNA和蛋白表达水平下调,同时抑制细胞G/S期转换。IFN-α诱导HBVAF中IFI16 mRNA和蛋白表达上调,抑制细胞G/S期转换,促进细胞凋亡。但在转染IFI16 siRNA的HBVAF中IFN-α的上述作用受到了抑制。结论 IFN-α抑制HBVAF增殖,促进其凋亡可能与促进IFI16表达有关。

Aim To investigate the effect of interferon-inducible protein 16（ IFI16） siRNA on the proliferation and apoptosis of interferon-α（ IFN-α） induction of human brain vascular adventitial fibroblasts（ HBVAF）. Methods The siRNA of IFI16 gene was transfected into HBVAF in vitro. 48 hours after transfection,the cells were exposed to 2 ×106U/L IFN-α for 24 h. Cell cycle and apoptosis were analyzed by flow cytometry. The mRNA and protein levels of IFI16 were measured by real-time PCR and Western blot. Results After transfection with IFI16 siRNA,the expression of IFI16 mRNA and protein levels was decreased in HBVAF,and the cell cycle at G/S transition was promoted.Meanwhile,stimulated with IFN-α up-regulated the expression of IFI16 mRNA and protein levels,and inhibited the cell cycle transition at G/S and promoted cells apoptosis in HBVAF. Such effect was restrained by transfection with IFI l6 siRNA into HBVAF. Conclusion IFN-α may inhibit cells proliferation and promote cells apoptosis of HBVAF by upregulating the expression of IFI16.