应用普通PCR与实时荧光标记PCR技术检测玉米子粒中转基因成分的比较

Comparison of the Analysis Methods Between Ordinary PCR and Real-time Fluorescent Mark PCR Technology to Detect Genetically Modified Ingredients in Maize Kernels

对比普通PCR法和实时荧光标记PCR法检测玉米子粒中转基因成分的检测效率,外源基因选取调控元件Ca MV35S启动子、NOS终止子、BAR、Cry IA(b)基因。普通PCR法在进行PCR扩增反应、电泳检测和凝胶成像后,进行结果判定;实时荧光标记PCR法的结果数据由Ct值判定,在一定范围内符合要求的数据即判定有效。通过两种方法比较,与普通PCR方法相比,实时荧光标记PCR法具有检测时间短、检测效率高、对环境造成的污染小等特点。

Using ordinary PCR method and real-time fluorescence mark method to detectgenetically modified ingredients in maize kernels. Exogenous gene selection has certain typical Ca MV35 S promoters and NOS terminator, BAR, Cry IA（b） gene. For the result verdict the ordinary PCR method is after PCR amplification reaction, electrophoresis inspection and gel imaging. While for the real-time fluorescent mark PCR method the result data will be judged by Ct value, data to be determined effective within a certain range meeting the requirements. By comparing the two methods, real-time fluorescent mark PCR method for qualitative analysis without preparation of standard curve, obviously saving time. This method isefficient and accurate, and less pollution to the environment.