海伦兜兰的无菌播种与快速繁殖

Aseptic seeding and rapid propagation of Paphiopedilum helenae

本文研究了海伦兜兰(Paphiopedilum helenae)的无菌播种与快速繁殖,结果表明:提高海伦兜兰种子萌发率较好的预处理方法是用0.1 mol·L^(-1) KOH处理10min;最适合海伦兜兰种子萌发的培养基为1/4MS+0.1%(m/V)活性炭+60 g·L^(-1)香蕉(Musa nana),种子萌发率达69.4%;最适合丛生芽诱导的培养基为1/2MS+0.5 mg·L^(-1) 6-苄氨基嘌呤(6-BA)+2.0 mg·L^(-1)萘乙酸(NAA)+0.1%活性炭+60 g·L^(-1)香蕉;最适合生根壮苗的培养基为MS+60 g·L^(-1)马铃薯(Solanum tuberosum)或MS+30 g·L^(-1)香蕉。

In this study,we investigated aseptic seeding and rapid propagation of Paphiopedilum helenae.The results show that the best method for pretreatment of P.helenae seeds was to soak them into 0.1 mol·L-1 KOH for 10 min;the best medium for seeds germination was 1/4MS＋0.1%（m/V） activated carbon（AC）＋ 60 g·L-1banana（Musa nana）,and the germinating rate was up to 69.4%;the best medium for multiple shoots induction was 1/2MS＋0.5 mg·L-1 6-benzylaminopurine（6-BA）＋2.0 mg·L-1 1-naphthaleneacetic acid（NAA）＋0.1% AC＋60 g·L-1 banana;the best medium for rooting and growth of plantlet was MS＋60g·L-1 potato（Solanum tuberosum） or MS＋30 g·L-1 banana.