土贝母正丁醇部位化学成分研究

Study on chemical compound of n-butyl alcohol fraction from Bolbostemma paniculatum(Maxim.)Franquet

采用薄层色谱,正相、反相硅胶,葡聚糖凝胶,半制备高效液相柱对土贝母正丁醇部位进行了分离研究.分离得到10个化合物,分别为:6-C-葡萄糖-5,7,3′,4′-四羟基黄酮(1),胡萝卜苷(2),胡萝卜苷棕榈酸酯(3),豆甾醇(4),β-谷甾醇(5),β-谷甾醇棕榈酸酯(6),正丁基-β-D-吡喃果糖苷(7),大黄素(8),大黄素甲醚(9),麦芽酚(10).除化合物(8)外,其余化合物都是首次从该植物中分离得到.

In the present work,thin layer chromatography（TLC）,normal-phase silica gel column,reveres-phase silica gel column,Sephadex-LH,semi-preparative HPLC column were used to isolate and analyze the chemical compositions of n-butyl alcohol fraction from Bolbostemma paniculatum（Maxim.）Franquet.Ten compounds were obtained and identified as：6-C-glucose-5,7,3′,4′-tetrahydroxy flavone（1）,Daucossterol（2）,Daucosterol palmitate（3）,Stigmasterol（4）,β-sitosterol（5）,Sitosteryl palmitate（6）,n-butyl-β-D-fructopyranoside（7）,Emodin（8）,Emodin monomethyl ether（9）,and Maltol（10）.Except for compound 8,the compounds were isolated for the first time from this plant.