银杏酚C15∶1与鲱鱼精DNA相互作用的光谱研究

Spectroscopic studies on the binding of Ginkgol C15∶ 1 and Herring Sperm DNA

目的:研究银杏酚C15∶1与DNA的相互作用情况。方法:吖啶橙(AO)为荧光探针,在293 K和310 K p H7.4的Tris-HCl缓冲液中,采用荧光光谱法、粘度法和热溶解实验研究银杏酚C15∶1和鲱鱼精DNA的相互作用方式。结果:银杏酚C15∶1与AO-DNA之间的猝灭方式为静态猝灭,根据热力学参数确定作用力类型是以氢键作用为主,判断银杏酚C15∶1与AO-DNA之间的作用方式主要是嵌插作用。结论:粘度法及热变性实验结果进一步证明银杏酚C15∶1与AO-DNA之间的主要作用方式是嵌插模式。

Objective： To study the interaction between Ginkgol C15：1 and Herring Sperm DNA. Methods： The interaction between Ginkgol C15：1 and Herring Sperm DNA was investigated by fluorescence spectroscopy, viscosity measurements and thermal dissolution assay in the Tris-HCI buffer（ pH7.4） at 293 K and 310 K,Acridine Orange（AO） as the fluorescent probe.Results：The fluorescence quenching of Ginkgol C15： 1 by AO-DNA was a static quenching procedure. The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicated the interaction between Ginkgol C15：1 and AO-DNA was intercalation.Conclusions： The results of viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15：1 and AO-DNA.