植物乳杆菌HLX37降胆固醇活性研究

Cholesterol-lowering activity of Lactobacillus plantarum HLX37 in vitro and in vivo

HLX37是从福建南平地区农户自然发酵的酸乳中分离得到的一株植物乳杆菌,本文对该菌株的耐酸耐胆盐能力和体内外降胆固醇活性进行研究。结果表明,HLX37菌株在p H为3.0和胆盐浓度为3.0 g/L环境下培养4 h的存活率分别为59.1%±4.8%和48.2%±4.9%,且对添加胆盐的MRS+CHOL培养基中胆固醇具有良好的降解能力(24 h和48 h降解率分别达37.33%±2.55%和44.72%±2.74%);连续灌胃HLX37发酵酸奶65 d可显著降低高胆固醇血症大鼠血清总胆固醇含量(p<0.05),而将该酸奶灭活后,其降胆固醇效果显著下降(p<0.05),说明HLX37活菌能够降低高胆固醇血症大鼠血清胆固醇水平。综上,HLX37菌株在体内外均显示出良好的降胆固醇活性,具有较大潜力应用于功能性发酵食品的生产中。

Lactobaci//us plantarum HLX37 is a new lactic acid bacteria strain isolated from the naturally fermented yogurt prepared by local dairy farmer in Nanping City（ Fujian, China）.ln this paper,the tolerance capacity of HLX37 to acid or bile salt stresses and its cholesterol-lowering activity both in vitro and in vivo were investigated.The result of in vitro study showed that HLX37 could survive after 4 h of incubation in MRS medium at pH3.0 or 3.0 g/L of bile salt,with the survival rates being 59.1% ±4.8% and 48.2% ±4.9%, respectively.HLX37 could reduce the cholesterol content by 37.33% ± 2.55% and 44.72% ± 2.74% after 24 h and 48 h of incubations in MRS medium supplemented with cholesterol and bile salt.The in vivo experiment demonstrated that intragastric administration of HLX37-fermented yogurt for consecutive 65 days could significantly decrease the serum level of total cholesterol in hypercholesterolaemic rats（p 〈 0.05）, while the heat-inactivated HLX37-fermented yogurt did not possess the ability. This indicated that only the active HLX37 strain had the capacity to lower serum cholesterol in hypercholesterolemic rats.in conclusion,HLX37 exhibited a good cholesterol-lowering activity both in vitro and in vivo, it may have great potentiality to be applied in the production of functional fermented foods.