托盘包装鲟鱼中腐败希瓦氏菌和总菌的生长动力学及货架期预测

Growth kinetics of Shewanella putrefaciens and total viable bacteria in pallet-package sturgeon and shelf life prediction

为研究托盘包装鲟鱼中特定腐败菌腐败希瓦氏菌和总菌的生长规律,用不同的微生物生长模型进行拟合,以此为基础建立并评价了货架期预测模型。以修正的Gompertz方程为一级模型,平方根方程为二级模型,建立腐败希瓦氏菌和总菌在0-20℃的生长预测模型和货架期预测模型。进一步通过托盘包装鲟鱼片在8℃和波动温度下贮藏数据对模型进行验证,结果显示腐败希瓦氏菌生长预测模型的准确度Af为1.28、1.35,偏差度Bf为0.91、1.08,货架期预测相对误差为5.23%、3.83%;而总菌的生长预测模型的Af为1.45、1.30,Bf为0.92、0.96,货架期预测相对误差为-4.40%、2.02%。以上结果表明根据两类微生物生长动力学建立的货架期预测模型对0~20℃贮藏的托盘包装鲟鱼货架期预测效果好,具有一定的实用价值。

The objective of this study was to investigate the growth kinetics of Shewanellaputrefaciens（specific spoilage organisms,SSO）and total viable count（TVC）in pallet-package sturgeon and develop and evaluate the predictive models of shelf life of pallet-package sturgeon.Modified Gompertz equation and square root equation were used as primary and secondary models respectively so as to fit the bacterial growth and shelf life prediction at temperatures from 0 ℃ to 20 ℃. Meanwhile,these models were validated under isothermal （8 ℃） and dynamic conditions.The accuracy factors （Af）, bias factors （ Bf ） and the relative errors of shelf life predictive models of Shewanella putrefaciens were 1.28 and ].35,0.9] and ].08,5.23% and 3.83%, respectively.Meanwhile, Af, Bf, and the relative errors of shelf lives predictive models in TVC were 1.45 and 1.30,0.92 and 0.96,-4.40% and 2.02%, respectively.The results indicated that the shelf life predictive models of pallet-package sturgeon stored at 0-20 ℃ based on the growth kinetics of Shewanella putrefaciens and TVC were effective and had great potential for application in pallet-package sturgeon product.