摘要
以‘东林瑞雪’‘夺目玛瑙’‘黄金盏’‘金不换’4种露地菊叶片外植体为试验材料,在MS基本培养基上添加不同浓度的6-BA、NAA进行高效稳定的再生体系的建立。结果表明:‘东林瑞雪’叶片最适分化培养基为MS+0.5mg·L^(-1) 6-BA+1.5mg·L^(-1) NAA;‘夺目玛瑙’叶片最适分化培养基为MS+1.0mg·L^(-1) 6-BA+2.0mg·L^(-1) NAA;‘黄金盏’叶片最适分化培养基为MS+0.5mg·L^(-1) 6-BA+1.0mg·L^(-1) NAA;‘金不换’叶片最适分化培养基为MS+1.0mg·L^(-1)6-BA+1.5mg·L^(-1) NAA;4种露地菊的最适生根培养基均为1/2MS培养基。
This experiment was conducted to construct a high efficient and stable regeneration system by adding different concentration of 6-BA and NAA to the basic MS medium with the leafs explant of four kinds of Chrysanthemum morifolium ,including ＇Donglinruixue＇ ＇Duomumanao＇ ＇Huangjinzhan＇ and ＇Jinbuhuan＇. The results showed that the optimum concentration combination of NAA and 6-BA for ＇Donlinruixue＇ was MS＋0. 5 mg·L^-1 NAA＋1. 5 mg ·L^-1 6-BA;for＇Duomumanao＇ was MS＋1. 0 mg ·L^-1 6-BA＋2.0 mg·L^-1 NAA; ＇Huangjinzhan＇was MS＋0. 5 mg·L^-1 6-BA＋1. 0 mg ·L^-1 NAA;＇Jinbuhuan＇ was MS＋1.0 mg·L^-1 6-BA＋1. 5 mg·L^-1 NAA;and the rooting medium of all the four kinds of Chrysanthemum mori morifolium was 1/2 MS medium.
出处
《北方园艺》
CAS
北大核心
2016年第19期119-124,共6页
Northern Horticulture
基金
"十二五"农村领域国家科技计划资助项目(2013AA102706)
