植物生长调节剂和蔗糖浓度对狼爪瓦松愈伤组织生物量及有效物质积累的影响

Effect of Plant Growth Regulators and Sucrose Concentrations on the Proliferation,Growth and Effective Compounds Content of Orostachys cartilaginous Callus

以狼爪瓦松愈伤组织为试材,采用正交实验的方法,研究了MS基本培养基浓度及6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)2种植物生长调节剂对狼爪瓦松愈伤组织生物量和有效物质含量的影响;同时,对适宜的蔗糖浓度进行了筛选。结果表明:MS+3.5mg·L^(-1) 6-BA+0.1mg·L^(-1) NAA组合,最有利于愈伤组织生长和多糖、总酚及黄酮的积累;MS+3.5mg·L^(-1)6-BA+0.1mg·L^(-1) NAA培养基中加入30g·L^(-1)的蔗糖时,愈伤组织生长最佳,有效物质含量最高,在此蔗糖浓度下狼爪瓦松愈伤组织可生产711.2mg·L^(-1)多糖、165.1mg·L^(-1)总酚和154.5mg·L^(-1)黄酮。

Orostachys cartilaginous callus was used as experiment material, the effect of MS strength and concentrations of plant growth regulators, 6-BA and NAA on callus biomass and effective compound accumulation was explored by orthogonal experiment method. The suitable sucrose concentration was also selected. The results showed that, MS medium supplemented with 3. 5 mg·L^-1 6-BA and 0. 1 mg·L^-1 NAA was favorable for callus growth and effective compound accumulation, the maxium fresh and dry weigth of calluses were found when the medium was supplied 30 g ·L^-1sucrose,711. 2 mg ·L^-1 of polysaccarides,165. 1 mg·L^-1 of phenolics and 154. 5 mg·L^-1of flavonoids were obtained in Orostachys cartilaginous callus.