低温层积过程中野鸦椿种子生理生化变化的研究

Biochemical and physiological changes of Euscaphis japonica seeds during the period of stratification

通过对低温层积不同时间野鸦椿种子内含物质变化情况,及不同播前预处理种子发芽情况的研究,得出如下结论：（1）野鸦椿种子不通过低温层积处理无法萌发。（2）野鸦椿种子在层积过程中主要通过大分子油脂类物质水解作种子萌发的物质动员。（3）野鸦椿种子通过低温层积的方法解除休眠的时间应控制在90～120 d内,层积时间过长发芽率反而下降。这与同属植物圆齿野鸦椿种子的萌发特性一致。（4）层积90～120 d时种子内可溶性糖含量有较大幅度下降,同时在120 d时种子的发芽率达到峰值,表明可溶性糖是野鸦椿种子萌发的直接利用能源物质。

Changes of reserved nutrients in Euscaphis japonica seeds during the process of stratification were studied, and seed germination characteristics after different pretreatments were investigated in this paper. The results were shown as following： （1）Seeds are unable to germinate under no low temperature stratification. （2）Seed germination of Euscaphisjaponica were primary mobilized by hydrolysis of macromolecular grease-like substance in the process of stratification. （3） The stratification time should be controlled within the 90 - 120 d, longer stratification time would make the rate of germination declined. The conclusions was the same as the result of Euscaphis konishii Hayata seeds, which belongs to the same genus as Euscaphisjaponica seeds. （4）During the stratification period of 90- 120 d, the content of soluble sugar declined significantly, as the same time, seeds germination rate arrived at peak in the 120 d, showing that soluble sugar was directly used for energy source of seed germination.