豚鼠电刺激频率跟随反应初步研究

Electrically-Evoked Frequency-Following Response(EFFR) of Guinea Pigs

目的探索豚鼠听觉系统对电刺激信号是否存在频率跟随现象。方法经圆窗龛使用纯音和白噪音电信号对豚鼠耳蜗行电刺激,记录其头皮电反应,经Matlab信号叠加和伪迹去除,计算EFFR信号相对幅度、输入输出函数,处死豚鼠和使用电阻代替豚鼠后记录EFFR变化验证EFFR来源。结果纯音电信号可诱发具有明显的周期性的EFFR信号,在797Hz和1597Hz所记录EFFR到的相对幅度(RA)均值明显高于白噪音RA均值(P<0.05);797Hz纯音电信号输出幅度随着刺激电刺激幅度增长呈非线性改变,呈现神经反应特有的阈值特性与饱和特性;豚鼠死亡前后EFFR的RA值差异具有高度显著性(P<0.001),豚鼠EFFR的RA值与使用电阻记录的RA值差异具有显著性(P<0.05)。结论听力正常豚鼠听觉系统对电刺激信号存在频率跟随现象。

Objective To explore the Electrically-Evoked Frequency-Following Response whether exist in guinea pig auditory system. Methods Periodic signals and white noise electrical stimuli were used for guinea pigs cochlear electrical stimulation, The EFFR were recorded in the guinea pigs scalp and analyzed, the EFFR also verified by recording of death guinea pigs and electrical resistance. Results EFFR evoked by periodic signals were significantly higher than those evoked by the white noise;the responses decreased significantly after death of the guineapigs; and the responses decreased significantly when the animal was replaced by an electrical resistance. Conclusions Electrically-Evoked Frequency-Following Response can be recorded in the guinea pigs with normal hearing.