采用高光谱成像仪对拔罐罐斑颜色光谱的初步观察

Color spectrum of cupping mark detected by hyperspectral camera:apreliminary observation

目的：对拔罐后局部皮肤的颜色变化进行定量观察,探索客观定量测量拔罐皮肤反应的方法。方法：选取健康受试者7人,使用定量可调的经络罐通仪于受试者背部分别进行4种强度负压的拔罐各5min,采用高光谱成像仪采集拔罐前与拔罐后即刻的皮肤光谱数据,并用数码照相机观察记录拔罐前后皮肤颜色变化。结果：拔罐前人体背部不同区域的光谱之间差异无统计学意义（P〉0.05）,其平均光谱在540~550nm及580~590nm处有两个峰。使用不同压力拔罐后,皮肤的光谱发生了变化,在-0.02MPa时,波长为550nm处下降最明显（-12.1%,P〈0.05）;在-0.03 MPa时,波长为540nm处下降幅度最大（-22.1%,P〈0.05）;在-0.04 MPa时,于610nm处有显著下降（-26.7%,P〈0.05）;在-0.05 MPa处,则多个波长处都有显著下降（均P〈0.05）。结论：不同负压拔罐后,皮肤的光谱可发生显著变化,且不同压力时最大变化处的波长有所不同,用高光谱仪可以定量地观察拔罐的皮肤颜色反应。

Objective To perform quantitative observation on the color change of local skin after cupping,so as to explore objective and quantitative methods for skin response of cupping.Methods Seven health subjects were included.By quantitative meridian cupping instrument,cupping methods with four types of pressures were respectively performed on subjects for 5min.The spectrum of cupping mark before and after the cupping was collected by hyperspectral camera,and the color change was recorded by digital camera.Results Before the cupping,the differences of back skin areas were not significant（P0.05）,and its average spectrum indicated two peaks at 540-550 nm and 580-590 nm.After cupping with different pressures,spectrum changes of skin were observed.For-0.02 MPa,the most significant reduction was observed at 550nm（-12.1%,P0.05）;for-0.03 MPa,the most significant reduction was observed at 540nm（-22.1%,P0.05）;for-0.04 MPa,the most significant reduction was observed at 610nm（-26.7%,P0.05）;for-0.05 MPa,the most significant reduction was observed at several spectrums（all P0.05）.Conclusion After cupping with different negative pressures,significant changes of spectrum are observed on skin;for different pressures,the spectrums of the most significant changes are different;the hyperspectral camera could be applied to perform quantitative observation on the color change of local skin.