人参皂苷Rb1对脑缺血/再灌注损伤大鼠脑血流量的影响

The effect of ginsenosides Rb1 on cerebral blood flow of cerebral ischemia/reperfusion injury in rats

目的：观察人参皂苷Rb1对缺血/再灌注（I/R）损伤大鼠脑血流量的影响，为探讨其脑保护机制提供新的理论基础。方法将24只大鼠按随机数字表法分为假手术组、模型组、生理盐水对照组、人参皂苷Rb1组，每组6只。采用大脑中动脉闭塞（MCAO）法复制大鼠脑I/R模型。人参皂苷Rb1组于制模后即刻腹腔注射人参皂苷Rb140 mg/kg，生理盐水对照组腹腔注射等体积生理盐水。脑I/R 24 h时监测大鼠局部脑血流量，观察大鼠行为学评分,并采用2，3，5-氯化三苯基四氮唑（TTC）染色法测量脑梗死体积。结果与假手术组比较，模型组梗死体积百分比〔（64.23±8.12）%比0%〕和神经行为评分〔分：3.0（2.0～4.0）比0（0～0），P＜0.05〕均明显升高，局部脑血流量明显减少（mL/min：125.75±57.65比225.01±78.25，P＜0.05）；与模型组和生理盐水对照组比较，人参皂苷Rb1组梗死体积百分比〔（23.62±8.74）%比（64.23±8.12）%、56.72±8.92〕和神经行为学评分〔分：0.5（0.0～2.0）比3.0（2.0～4.0）、3.5（1.0～4.0）〕均明显降低（P＜0.05），局部脑血流量明显增多（mL/min：177.25±75.36比125.75±57.65，132.65±58.65，P＜0.05）。结论人参皂苷Rb1可以增加I/R大鼠脑血流量，这可能是人参皂苷Rb1发挥脑保护作用的机制之一。

ObjectiveTo observe the effect of ginsenosides Rb1 on cerebral blood flow of rat models with cerebral ischemia/reperfusion （I/R） injury, which could provide a new theory of cerebral protective mechanism about ginsenosides Rb1.Methods Twenty-four rats were randomly divided into sham-operation group, model group, normal saline control group and ginsenosides Rb1 group, 6 rats in each group. The middle cerebral artery occlusion （MCAO） model was established by thread embolism method. At the end of I/R, in the rat of ginsenosides Rb1 group, ginsenosides Rb1 40 mg/kg was immediately intraperitoneally injected, while in the rat of normal saline control group, an equal volume of normal saline was injected intraperitoneally. After I/R for 24 hours, the cerebral local amount of blood flow was measured, the rats＇ behavior score was observed, and the volume of cerebral infarction was monitored by 2, 3, 5-triphenyl tetrazolium chloride （TTC） staining.Results The percentage of volume of cerebral infarction [（64.23±8.12）% vs. 0%] and behavior score [3.0 （2.0-4.0） vs. 0 （0-0）,P〈 0.05] in model group were significantly higher than those in sham-operation group, while the cerebral local amount of blood flow in model group was obviously lower than that in sham-operation group （mL/min： 125.75±57.65 vs. 225.01±78.25,P〈 0.05）; Compared with the model group and normal saline control group, the percentage of volume of cerebral infarction [（23.62±8.74）% vs. （64.23±8.12）%, 56.72±8.92] and behavior score [0.5 （0.0-2.0） vs. 3.0 （2.0-4.0）, 3.5 （1.0-4.0）] in the ginsenosides Rb1 group were significantly lower, the cerebral local amount of blood flow was markedly increased in the ginsenosides Rb1 group （177.25±75.36 vs. 125.75±57.65, 132.65±58.65,P〈 0.05）.Conclusion Ginsenosides Rb1 can increase the cerebral blood flow in rats with cerebral I/R injury, which maybe one of the mechanisms of cerebral protection of Ginsenosides Rb1.