同期排卵-定时输精对产后难配泌乳奶牛繁殖效率的影响

Effects of Ovsynch-Timed Artificial Insemination on Reproductive Performance in Infertility Holstein Milking Cows

本试验旨在研究同期排卵-定时输精技术对产后难配泌乳奶牛繁殖效率的影响。选择88头健康、膘情适中、产后不发情或久配不孕(难配)的荷斯坦泌乳奶牛,随机分为3组,分别按照Ovsynch、CIDR+Ovsynch或CIDR+PG+Gn RH程序进行激素处理和定时人工授精,CIDR+Ovsynch或CIDR+PG+Gn RH试验组分别在埋栓、撤栓、第二次注射Gn RH和定时输精时采集10头奶牛血样,测定血清中孕酮(P4)和雌激素(E2)含量。结果表明:3种同期排卵-定时输精程序处理后都可以使一定数量的难配泌乳奶牛配种妊娠,且3种程序对产后难配泌乳奶牛人工授精后的不返情率无显著影响(P>0.05);CIDR+Ovsynch试验组泌乳奶牛人工授精后的情期受胎率(46.7%)高于Ovsynch组(28.6%)和CIDR+PG+Gn RH组(26.7%),但差异不显著(P=0.08,P=0.06);CIDR+Ovsynch或CIDR+PG+Gn RH程序处理过程中,泌乳奶牛血清中P4含量的变化趋势一致,E2含量的变化趋势因程序开始处理时奶牛所处的发情周期阶段不同而不同,但两种程序配种妊娠奶牛定时输精时血清中E2含量均极显著高于配种未妊娠奶牛(P<0.01)。因此,同期排卵-定时输精技术可用于产后难配泌乳奶牛的配种处理。

This experiment was carried out to study the effects of Ovsynch-timed artificial insemination （TAI） on reproductive performance in infertility cows. A total of 88 Holstein milking cows （healthy, medium somatotype）, which were difficult to mate, were randomly assigned into three groups. The experimental cows were treated with Ovsyneh, CIDR＋Ovsynch or CIDR＋PG＋GnRH program, respectively. Blood samples were collected from 10 cows in CIDR＋ Ovsynch and CIDR＋PG＋GnRH treatment group at CIDR insertion, CIDR remove, the second GnRH injection and AI to determine the concentration of P4 and E2 in serum. The results showed that the three TAI programs could induce a certain number ofinfertility cowspregnancy, and there was no significant difference in the non-return rate between the three groups （P〉0.05）. The conception rate of the CIDR＋Ovsynch group （46.7%） had a tendency to be significantly higher than that of the Ovsynch group （28.6%） and the CIDR＋PG＋GnRH group （26.7%）, but there also was no significant difference （P=0.08, P=0.06）. According to the determination results, the trend of the concentration of P4 in serum of the CIDR ＋Ovsynchgroup was similar to that of the CIDR ＋PG ＋GnRH group, but the trend of the concentration of E2 in serum depend on the stage of estrous cycle before the beginning of TAI programs. The concentration of E2 in serum of pregnant cows was very significantly higher than that of non-pregnant cows （P〈0.01）. In consequence, Ovsynch-timed artificial insemination could be used for the infertilitycows.