江苏省南京地区幽门螺杆菌感染及耐药性调查研究

Research on the infection and medicine-resistance of Helicobacter pylori in Jiangsu Province Nanjing District

[目的]调查幽门螺杆菌(Hp)在江苏南京地区人群中分布情况及其耐药性,为临床合理选择抗生素提供依据。[方法]选择慢性胃炎、消化性溃疡共321例患者,取胃黏膜活检组织在微需氧环境中培养Hp,对培养的190株Hp作药物敏感性试验(K-B药敏纸片法)。[结果]Hp培养阳性率依次为消化性溃疡(76.7%)、慢性胃炎(48.8%)。190例Hp菌株的药物敏感性试验结果显示对甲硝唑耐药率60.5%、对克拉霉耐药率29.5%、对左氧氟沙星耐药率14.7%、对庆大霉素耐药率11.6%、对阿莫西林耐药率7.4%、对呋喃唑酮耐药率5.8%。[结论]本地区Hp感染者对阿莫西林、呋喃唑酮耐药率较低,可指导临床选择用药。

[Objective]To investigate the infection and drug-resistance of Helicobacter pylori（Hp）in Jiangsu Province Nanjing District.[Methods]The Bacteria were separated and cultured from the patients＇ gastric mucosa tissue.Suspected bacteria clones were smeared,stained and microscopic examined,and biochemistically tested for Hpidentification.The Hppositive clones were also tested for drug-sensitivity by K-B methods.[Results]The total of 321 speciment from patients＇ gastric mucosa tissue were examined in Jiangsu Province Nanjing District were collected.190 Hpclones were identified.It showed that the Hp positive rate in gastritis was 48.8%,in ulcer was 76.7%.Medicine sensitivity test were performed on the190 Hp,the results indicated：the drug resistance rate to metronidazole was 60.5%,kalamycin was 29.5,levofloxacin was 14.7%,gentamycin was 11.6%,amoxicillin was 7.4% and furazolidone was 5.8%.[Conclusion]The infected Hpin the patients in Jiangsu Province Nanjing District is sensitive to amoxicillin,furazolidone,which provide the evidence for the treatment of Hpin the area.