GPER介导雌激素对兔耳增生性瘢痕的影响

Influences of estrogen mediated by GPER on hypertrophic scar in rabbit ears

目的观察G蛋白偶联雌激素受体(GPER)对于兔耳增生性瘢痕的影响。方法选取36只新西兰大耳兔建立模型,随机分为6个组,分别为E2组(17-β-雌二醇)、G1组(雌激素受体激动剂)、E2+G15组(17-β-雌二醇+雌激素受体拈抗剂)、G1+G15组、DMSO组(溶剂)和空白对照组。于兔耳根部皮下持续注射给药15 d后切取标本,观察各组瘢痕增生情况。瘢痕增生指数(SEI)、成纤维细胞数量、胶原纤维排列以及通过实时定量PCR检测TGF-β1、collagenⅠ、collagenⅢ和GPER的mRNA表达情况。结果通过大体观察及显微镜检发现,E2和G1组相比空白对照组的瘢痕增生明显,成纤维细胞数量明显增多,胶原排列紊乱;E2和G1组经G15处理后,瘢痕明显缩小;检测并分析数据SEI以及TGF-β1、collagenⅠ、collagenⅢ和GPER的mRNA表达量,E2和G1组相对于空白对照组均明显升高(P<0.05),而E2和G1组经G15处理后则显著降低(P<0.05);DMSO组相对于空白对照组,其差异无统计学意义(P>0.05)。结论GPER介导雌激素对于兔耳增生性瘢痕具有促进作用。

Objective To observe the influences of G protein-coupled estrogen receptor （GPER） on hypertrophic scar of rabbit ears.Methods Thirty-six New Zealand white rabbits were chosen to establish the models of hyperplastic scar in rabbit ears.Rabbit models were randomly assigned to 6 groups,in which E2,G1,E2 ＋ G15,G1 ＋ G15,DMSO and blank control groups;Specimens were taken at 15 days after receiving subcutaneous injection at the ear roots of the rabbits and observations were made in each group.The scar elevation index （SEI）,fibroblasts quantity,orientation of collagen fiber and real-time quantitative PCR detected the expressions of TGF-β1,collagen Ⅰ,collagen Ⅲ and GPER mRNA.Results Compared with the blank control group,the scar hyperplasia in the E2 and G1 groups was obvious with more fibroblasts and higher collagen density with irregularly arrangement;that in the E2 and G1 groups significantly reduced after G15 treatment by gross observation and microscopic examination.The SEI and the expression of TGF-β1,collagenⅠ,collagen Ⅲ,and GPER mRNA were significantly higher in those in the E2 and G1 groups compared with the blank control group （P＜0.05）,which were lower in the E2 and G1 groups treated with G15 treatment （P＜0.05）;the DMSO group.The blank control group had few obvious differences （P＞ 0.05）.Conclusion Estrogen mediated by GPER has a promoting effect on hypertrophic scar in rabbit ears.