Dermcidin在原发性肝癌诊断及病情评估中的意义

Significant of Dermcidin in the diagnosis of hepatocellular carcinoma and metastasis

目的明确Dermcidin(DCD)作为原发性肝癌(HCC)生物标志物的可能性。方法 82例已确诊的HCC患者纳入肝癌组,其中转移组35例,未转移组47例。33例代偿期乙型肝炎肝硬化患者纳入肝硬化组,44例健康体检者纳入健康对照组。收集血清标本,通过ELISA法检测血清中DCD的水平,并采用电化学发光免疫分析法检测甲胎蛋白(AFP)水平。结果肝癌组血清中DCD、AFP水平均明显高于健康对照组及肝硬化组,差异有统计学意义(P<0.05)。转移组患者血清中DCD的水平明显高于未转移组,差异有统计学意义(P=0.001)。而AFP水平在转移组与未转移组间比较,差异无统计学意义(P=0.697)。DCD及AFP在不同性别和不同年龄的受试者间的表达差异无统计学意义(P>0.05)。结论 DCD有望成为HCC新的血清标志物,可判断肝癌是否发生转移,DCD在不同性别和不同年龄的受试者上的表达没有明显差异,具有较广的适用性。

Objective To explore the clinical application of serum Dermcidin（DCD）in the diagnosis of primary carcinoma of liver（HCC）.Methods A total of 82 HCC patients were recruited into HCC group,35 HCC patients with tumor metastasis,47 HCC patients without tumor metastasis.33 patients in hepatic cirrhosis group,44 healthy individuals were recruited into control group.Serum samples were collected,then detected DCD by ELISA,and measured AFP levels by electro chemiluminescence immunoassay（ECLIA）.Results Serum DCD concentrations in HCC group were significant higher than those in hepatic cirrhosis group and control group（P〈0.05）.DCD in HCC patients with tumor metastasis was significant higher than that in HCC patients without tumor metastasis（P=0.001）,but there was no significant difference on AFP between them（P=0.697）.There were no significant difference on DCD and AFP among patients with different gender and different age（P〉0.05）.Conclusion DCD shows a high expression in serum of HCC patients,especially in HCC metastasis group.It might serve as a candidate biological marker for diagnosis and differential diagnosis of HCC.