青浦区水产品中副溶血性弧菌污染状况与分子分型特征

Study on the pollution status and molecular characteristics of Vibrio parahaemolyticus

目的了解副溶血性弧菌在青浦区部分市售水产品中的污染情况及分子分型。方法于不同季度,分别从酒店、农贸市场和大型超市采取鱼类、虾和贝类等标本,参照GB 4789.7,采用稀释培养计数(MPN)法进行副溶血性弧菌定量检测,并完成分离菌株毒力基因和脉冲场凝胶电泳(PFGE)分子分型的检测。结果 144份水产品中共检出副溶血性弧菌68份,阳性率为47.00%,虾类、贝类和鱼类中副溶血性弧菌几何平均数分别为465.2、291.0、146.1MPN/g;所检出菌株均不携带TDH和TRH 2种毒力基因,68株副溶血性弧菌PFGE共分为63种带型,呈现明显多态性。结论青浦区市售水产品中副溶血性弧菌污染较严重,应采取有效措施防止由水产品引起的副溶血性弧菌食源性疾病,该地区菌株间遗传同源关系较远。

Objective To explore the pollution status and mole cular characteristics of Vibrio parahaemolyticus in retail aquatic products in Qingpu District.Methods Samples of fish,shrimp and shellfish from the hotel,farmers market and super market in different seasons were collected,and then Vibrio parahaemolyticus was isolated and identified by MPN method according to the GB4789.7,the detection of the virulent genes and molecular typing of the isolated strains were completed.Results A total of 68 strains of Vibrio parahaemolyticus were detected among 144 samples of aquatic products,the positive rate was 47.00%.The geometric mean concentration of Vibrio parahaemolyticus in shrimp,shellfish and fish were 465.2,291.0,146.1MPN/g.All the detected strains didn′t carry the TDH and TRH virulent genes,68 strains were divided to 63 patterns by pulse alternative field gel electrophoresis（PFGE）method,which showed high polymorphic.Conclusion The Vibrio parahaemolyticus pollution is serious in retail aquatic products in Qingpu District,the monitoring on preventing the food poisoning and food origin disease caused by Vibrio parahaemolyticus should be strengthened,there is no homology relationship among the detected strains.