饮酒对单纯性肥胖大鼠肝脏功能的影响

Effect of drinking on liver function in nutritional obesity rats

目的:探究饮用不同浓度的白酒对单纯性肥胖大鼠肝脏功能的影响。方法:选取30只SD大鼠随机分为5组:对照组、单纯性肥胖模型组、20%白酒+单纯性肥胖模型组、40%白酒+单纯性肥胖模型组、60%白酒+单纯性肥胖模型组,每组6只。用高脂饲料喂养建立单纯性肥胖大鼠模型,并以不同浓度的白酒和生理盐水分别灌胃饲养1w,随后对各组大鼠心脏取血,制作血清样本,检测血清中谷丙转氨酶(Alanine aminotransferase,ALT)、谷草转氨酶(Aspartate transaminase,AST)含量,并取其肝脏制作HE染色切片。结果:病理切片显示20%、40%、60%白酒+单纯性肥胖模型组肝小叶周边细胞出现不同程度水肿、脂肪变、坏死。生化分析表明20%、40%、60%白酒+单纯性肥胖模型组DeRitis比值(AST/ALT)较空白对照组和单纯性肥胖模型组均有增高,且此三组的DeRisit比值随白酒浓度升高而增加。结论:20%、40%、60%白酒+单纯性肥胖模型组大鼠肝细胞有不同程度病理损伤。饮酒对单纯性肥胖大鼠肝脏的损伤具有剂量依赖性。

Objective：To study the effects of different concentrations of alcohol on liver enzymes and liver structure on nutritional obesity rats.Methods：Thirty SD rats were randomly divided into five groups：nutritional obesity rat model group,nutritional obesity rat model group with 20% alcohol intake,nutritional obesity rat model group with 40% alcohol intake,nutritional obesity rat model group with 60% alcohol intake,control group.The nutritional obesity rat model was established by feeding high-fat diet.Intragastric administration of different concentrations of alcohol and saline were performed for each group for 1week,then detecting AST and ALT value viacardiac blood sample,and liver histological slices were analysed.Results：From the pathological sections of nutritional obesity rat model group with 20%,40%,60% alcohol intake,it can be seen that varying degrees of celluar steatosis,edema,inflammation and necrosis of around the hepatic lobule occured.Biochemical analysis showed that the DeRitis ratios（AST/ALT）of nutritional obesity rat model group with 20%,40%,60% alcohol intake were significantly higher than that of nutritional obesity rat model group and normal control group,the DeRitis ratios（AST/ALT）of three groups increased with the increasing of the concentration of alcohol.Conclusion：Liver cells of rats in three models were damaged to different extent.The liver injury in rats drinking on nutritional obesity was dose dependent.