结直肠癌患者外周血中淋巴细胞亚群的改变

Change of peripheral blood lymphocyte phenotypes subsets in patients with colorectal cancer

目的探讨结直肠癌患者外周血中淋巴细胞亚群的表达变化及其与疾病发生发展的关系。方法选取148例结直肠癌患者(结直肠癌组)和267例体检人员(对照组),采用流式细胞技术检测外周血中淋巴细胞亚群CD^(4+)、CD^(8+)、CD^(19+)、CD^(25+)、CD4^(4+)及CD^(56+)等细胞计数比例,结合临床病理资料及随访数据进行分析。结果结直肠癌组患者外周血中CD8+比例高于对照组,CD^(4+)、CD^(19+)、CD4^(4+)比例与CD^(4+)/CD^(8+)比值低于对照组,差异均有统计学意义(t分别=-3.72、2.44、4.50、2.17、2.32,P均<0.05)。在结直肠癌组患者的亚组分析中,结直肠癌患者中CD^(8+)、CD^(56+)、CD^(8+)/CD^(28-)、CD^(4+)/CD^(8+)的变化与淋巴结转移和病理分期相关(t分别=-2.39、2.09、-3.08、2.67、-2.32、2.57、-2.73、2.34,P均<0.05)。结论结直肠癌患者外周血淋巴细胞亚群变化明显,患者的细胞免疫增强,而体液免疫和固有免疫受到抑制。

Objective To investigate the relationship between the change of peripheral blood lymphocyte subsets in patients with colorectal cancer and the influence on the disease development. Methods A total of 148 patients with colorectal cancer（CRC group） and 267 volunteers（control group） were selected to test the percentages of lymphocyte subsets distribution（CD4＋,CD8＋,CD19＋,CD25＋,CD44＋and CD56＋） by flow cytometry in peripheral blood. The clinical characteristics and follow-up data were combined to analyse with the outcome.Results The proportion of CD8＋in the CRC group were significantly higher than the control group,whereas the CD4＋,CD19＋,CD44＋and CD4＋/CD8＋were significantly lower（t=-3.72,2.44,4.50,2.17,2.32,P0.05）. In the subgroup analyzes of CRC patients,the change of CD8＋,CD56＋,CD8＋/CD28-,and CD4＋/CD8＋were significantly related to lymph node metastasis and pathological stages（t=-2.39,2.09,-3.08,2.67,-2.32,2.57,-2.73,2.34,P0.05）. Conclusion The peripheral blood lymphocyte subsets in patients with colorectal cancer are changed. The cellular immunity is enhanced and the humoral immunity/inherent immunity is suppressed relatively.